Discovery Research
Spend less time searching for a transfection reagent and more time moving your innovations forward.
We offer a range of effective transfection solutions and tools for a comprehensive set of cell lines. These solutions are intentionally designed to be gentle on cells and maximize cell delivery while using a simplified, straightforward protocol.
Combine the Mirus Bio portfolio with our team of dedicated technical support scientists and you can get answers to compelling biological questions with less stress and more confidence.
Have a question? Let’s talk.
Discovery Research Applications
TransIT-X2® Dynamic Delivery System
A novel, polymeric system for delivery of multiple nucleic acids to mammalian cells. Delivers CRISPR/Cas9 components in the following formats:
- DNA – Deliver plasmid DNA expressing Cas9 or guide RNA
- Guide RNA – Deliver guide RNA oligonucleotides targeting your gene of interest
- RNP – Deliver Cas9/guide RNA ribonucleoprotein complexes
TransIT®-mRNA Transfection Kit
A high efficiency, low toxicity transfection reagent for mammalian cells. Delivers CRISPR/Cas9 components in the following formats:
- mRNA – Deliver messenger RNA expressing Cas9
- Guide RNA – Deliver guide RNA targeting your gene of interest
Ingenio® Electroporation Solution
A high efficiency electroporation solution compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®. Delivers CRISPR/Cas9 components in the following formats:
- DNA – Deliver plasmid DNA expressing Cas9 or guide RNA
- mRNA – Deliver messenger RNA expressing Cas9
- Guide RNA – Deliver guide RNA oligonucleotides targeting your gene of interest
- RNP – Deliver Cas9/guide RNA ribonucleoprotein complexes
TransIT-X2® Dynamic Delivery System
An advanced system for delivery of CRISPR/Cas9 components in human IPSCs
- Broad Spectrum Delivery – High efficiency delivery in a wide range of cell types
- Genome Editing – Deliver Cas9 protein/guide RNA ribonucleoprotein (RNP) complexes
- Technology – Novel, non-liposomal, polymeric delivery
TransIT®-2020 Transfection Reagent
A high performance, animal-free, broad spectrum DNA transfection reagent
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
- Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
- Animal Origin Free – High performance with maximum compatibility
TransIT®-LT1 Transfection Reagent
A low toxicity, broad spectrum DNA transfection reagent
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
- Low Cellular Toxicity – Maintain cell density and reduce experimental biases
- High Efficiency Delivery – Achieve expression in a large population of cells
TransIT®-mRNA Transfection Kit
A high efficiency, low toxicity transfection reagent for large RNA delivery
- Low Cellular Toxicity – Maintain cell density and reduce experimental biases
- High Efficiency Delivery – Achieve RNA delivery in a large population of cells
- Serum Compatible – Perform transfections in the presence of serum which eliminates the need for a media change
Ingenio® Electroporation Solution
High efficiency electroporation using any instrument
- High Efficiency Electroporation – Deliver to hard to transfect cell lines and primary cells
- Compatible with Most Conventional Electroporation Devices – Use your existing system including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®
- Save Money Without Sacrificing Performance – Available as a stand-alone solution or as part of a complete kit with cuvettes and cell droppers
Broad Spectrum TransIT® Transfection Reagent Recommendations for Gene Silencing
| Nucleic Acid to be Transfected | Transfection Reagent Recommendation |
|---|---|
| siRNA/miRNA | Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT-TKO® Transfection Reagent Option 3: TransIT-siQUEST® Transfection Reagent |
| shRNA encoding Plasmid DNA libraries | Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT®-2020 Transfection Reagent Option 3: TransIT®-LT1 Transfection Reagent |
| siRNA/miRNA and Plasmid DNA | TransIT-X2® Dynamic Delivery System |
TransIT®-mRNA Transfection Kit
Each Kit is supplied with the TransIT®-mRNA Transfection Reagent and the mRNA Boost Reagent
- Low Cellular Toxicity – Maintain cell density and reduce experimental biases
- High Efficiency Delivery – Achieve RNA delivery in a large population of cells to ensure experimental success
- Serum Compatible – Perform transfections in the presence of serum which eliminates the need for a media change and maintains cellular health
- Deliver Various Sizes of RNA – Ideal for specialized applications, such as virus production, protein expression and CRISPR genome editing
TransIT-X2® Dynamic Delivery System
A high efficiency, low toxicity transfection reagent for large RNA delivery
- Efficiency –Exceptional broad spectrum transfection
- Versatility –Cutting edge delivery of plasmid DNA and small RNAs
- Technology – Novel, non-liposomal, polymeric delivery
TransIT®-LT1 Transfection Reagent
A low toxicity, broad spectrum DNA transfection reagent
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
- Low Cellular Toxicity – Maintain cell density and reduce experimental biases
- High Efficiency Delivery – Achieve expression in a large population of cells
TransIT®-2020 Transfection Reagent
A high performance, animal-free, broad spectrum DNA transfection reagent
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
- Gentle to Your Cells – Balances high efficiency nucleic acid deliver and low cellular toxicity
- Animal Origin Free – High performance with maximum compatibility
TransIT®-LT1 Transfection Reagent
- Broad Spectrum DNA Delivery – Utilize one transfection reagent and protocol for a variety of cells
- High Efficiency Delivery – Achieve expression in a large population of cells for experimental success
- Deliver Single or Multiple Plasmids – Suitable for applications such as virus production
- Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines and primary cells
- Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
- Animal Origin Free – High performance with maximum compatibility
Different TransIT® Transfection Reagents are recommended depending on the combinations of nucleic acids being delivered. All of the following reagent recommendations are of broad spectrum formulations that are chemically distinct. Depending on the cell type being transfected, one reagent may have superior performance over others; for cell-type specific recommendations, please consult the Reagent Agent® transfection database or contact techsupport@mirusbio.com
| Nucleic Acid(s) to be Co-transfected | Transfection Reagent |
|---|---|
| Multiple plasmid DNAs | Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT®-2020 Transfection Reagent Option 3: TransIT®-LT1 Transfection Reagent |
| Multiple siRNAs | Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT-TKO® Transfection Reagent Option 3: TransIT-siQUEST® Transfection Reagent |
| Plasmid DNA and siRNA | TransIT-X2® Dynamic Delivery System |
| Multiple mRNAs | TransIT®-mRNA Transfection Kit |
| Plasmid DNA and mRNA | TransIT-X2® Dynamic Delivery System and TransIT®-mRNA Transfection Kit |
| Multiple oligonucleotides | TransIT®-Oligo Transfection Reagent |
| Plasmid DNA and oligonucleotides | TransIT-X2® Dynamic Delivery System and TransIT®-Oligo Transfection Reagent |
| Other combinations | Contact techsupport@mirusbio.com |
Ingenio Electroporation: A Universal Solution
Ingenio® Electroporation Solution is compatible with multiple cell types and devices.
- Use your existing system including the Ingenio® EZporator®, Nucleofector®, Bio-Rad® or Harvard BTX®
- Reduce cost when using the Lonza Nucleofector® without sacrificing performance
Ingenio® EZporator Electroporation System
The Ingenio® EZporator® Electroporation System is an easy-to-use electroporation system for mammalian cells.
- Easily optimize electroporation parameters for each cell type
- Every system comes with a complimentary Ingenio® Electroporation Kit