We offer a range of effective transfection solutions and tools for a comprehensive set of cell lines. These solutions are intentionally designed to be gentle on cells and maximize cell delivery while using a simplified, straightforward protocol.
Combine the Mirus Bio portfolio with our team of dedicated technical support scientists and you can get answers to compelling biological questions with less stress and more confidence.
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A novel, polymeric system for delivery of multiple nucleic acids to mammalian cells. Delivers CRISPR/Cas9 components in the following formats:
A high efficiency, low toxicity transfection reagent for mammalian cells. Delivers CRISPR/Cas9 components in the following formats:
A high efficiency electroporation solution compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®. Delivers CRISPR/Cas9 components in the following formats:
An advanced system for delivery of CRISPR/Cas9 components in human IPSCs
A high performance, animal-free, broad spectrum DNA transfection reagent
A low toxicity, broad spectrum DNA transfection reagent
A high efficiency, low toxicity transfection reagent for large RNA delivery
High efficiency electroporation using any instrument
Nucleic Acid to be Transfected | Transfection Reagent Recommendation |
---|---|
siRNA/miRNA | Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT-TKO® Transfection Reagent Option 3: TransIT-siQUEST® Transfection Reagent |
shRNA encoding Plasmid DNA libraries | Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT®-2020 Transfection Reagent Option 3: TransIT®-LT1 Transfection Reagent |
siRNA/miRNA and Plasmid DNA | TransIT-X2® Dynamic Delivery System |
Each Kit is supplied with the TransIT®-mRNA Transfection Reagent and the mRNA Boost Reagent
A high efficiency, low toxicity transfection reagent for large RNA delivery
A low toxicity, broad spectrum DNA transfection reagent
A high performance, animal-free, broad spectrum DNA transfection reagent
Different TransIT® Transfection Reagents are recommended depending on the combinations of nucleic acids being delivered. All of the following reagent recommendations are of broad spectrum formulations that are chemically distinct. Depending on the cell type being transfected, one reagent may have superior performance over others; for cell-type specific recommendations, please consult the Reagent Agent® transfection database or contact techsupport@mirusbio.com
Nucleic Acid(s) to be Co-transfected | Transfection Reagent |
---|---|
Multiple plasmid DNAs | Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT®-2020 Transfection Reagent Option 3: TransIT®-LT1 Transfection Reagent |
Multiple siRNAs | Option 1: TransIT-X2® Dynamic Delivery System Option 2: TransIT-TKO® Transfection Reagent Option 3: TransIT-siQUEST® Transfection Reagent |
Plasmid DNA and siRNA | TransIT-X2® Dynamic Delivery System |
Multiple mRNAs | TransIT®-mRNA Transfection Kit |
Plasmid DNA and mRNA | TransIT-X2® Dynamic Delivery System and TransIT®-mRNA Transfection Kit |
Multiple oligonucleotides | TransIT®-Oligo Transfection Reagent |
Plasmid DNA and oligonucleotides | TransIT-X2® Dynamic Delivery System and TransIT®-Oligo Transfection Reagent |
Other combinations | Contact techsupport@mirusbio.com |
Ingenio® Electroporation Solution is compatible with multiple cell types and devices.
The Ingenio® EZporator® Electroporation System is an easy-to-use electroporation system for mammalian cells.