AAV Manufacturing

Increase AAV Titers and Percent Full Capsids Through Advanced Polymer and Lipid Technologies

Harnessing recombinant adeno-associated virus (AAV) as vectors for cell and gene therapy requires robust upstream manufacturing processes that are reproducible, scalable, and quality-controlled. By combining purpose-built polymers and lipids, Mirus Bio has developed animal origin free reagents that seamlessly blend into the AAV manufacturing process at the time of transfection to dramatically increase AAV titers. With scalable, consistent performance, the TransIT-VirusGEN® Transfection Reagent paired with the RevIT™ AAV Enhancer will improve the efficiency and output of your AAV production platforms, all while driving down the cost per dose.

AAV Manufacturing

“This is something that could definitely provide a cost-savings for a customer”

Achieve 2-4X Higher AAV Titer and Full Capsid Percentage

Our titer scale up compares very favorably to all of the other scales that we have tested. In addition, percent-full capsids scales the same as well, resulting in greater than 40% full in the 50 L bioreactor.

Chris Brown

Senior Scientist of Process Development

ReciBioPharm

Adeno-Associated Viral Vector Production

Improve AAV productivity and critical process parameters with a best-in-class transfection reagent and enhancer platform to ensure success in your AAV viral vector manufacturing:

Ready to Explore the Possibilities?

Schedule a meeting to discuss your needs and begin your journey to higher titers and percent full capsids.

How to Scale Your AAV Manufacturing Process

Scaling your AAV manufacturing process can be a complex endeavor, but with the right tools and support, it becomes a streamlined and efficient operation. Mirus Bio provides industry-leading solutions to enhance every step of your AAV production, ensuring high yields and consistent results. Here’s how our products and support can elevate your AAV platform:

AAV Transfection Reagent

Mirus Bio’s transfection reagents are designed to deliver higher titers across all AAV serotypes, media, and HEK cell lines. Our innovative formulations ensure optimal gene delivery, maximizing your output and efficiency. Our reagents provide reliable, high-performance results every time over polymer only reagents

TransIT VIrusGEN tin on a counter

AAV Enhancers

Increase your AAV titer up to 4X with RevIT™ AAV Enhancer. RevIT is compatible with multiple serotypes, media, and HEK cell lines, making it an easy and effective addition to your workflow. By incorporating RevIT into your process, you can achieve significantly higher yields with minimal effort.

Overcoming AAV Challenges

Utilize the most responsive and dedicated support team in the industry. Our team of transfection experts is committed to helping you succeed.

A dedicated team of Field Application Scientists (FAS) can help you plan your experiments, review your data, and provide on-site assistance, saving you time and resources.

Personalized support to optimize your processes, reducing the risk of bioreactor issues, wasted materials, lost time, or wasted budget.

Miguel and Becky optimizing an upstream process
a 200 liter bioreactor. It is easy to scale your viral vectors.

Scaling Up with GMP Manufacturing

Your drug assets require the support of GMP and ISO-compliant material, designed to enable the commercial-scale production. Choose reagents and enhancers that maintain their results from the smallest shake flasks to the largest bioreactors.

Through longer transfection complex formation times, your upstream processing team can easily scale up to larger bioreactors with products designed to reduce risks and cut costs.

Confidently scale your AAV production from the lab to commercial manufacturing.

Transfection Adoption Partnership

Join our Transfection Adoption Partnership (TAP) program to gain access to exclusive benefits, including:
  • Preferred pricing
  • Priority white glove service
  • Thought leadership opportunities
Partner AAV manufacturing companies receive tailored support to enhance their transfection processes, ensuring they remain at the forefront of AAV manufacturing innovation.

How to Scale Your AAV Manufacturing Process

Scaling your AAV manufacturing process can be a complex endeavor, but with the right tools and support, it becomes a streamlined and efficient operation. Mirus Bio provides industry-leading solutions to enhance every step of your AAV production, ensuring high yields and consistent results. Here’s how our products and services can elevate your AAV platform:

Mirus Bio’s transfection reagents are designed to deliver higher titers across all AAV serotypes, media, and HEK cell lines. Our innovative formulations ensure optimal gene delivery, maximizing your output and efficiency. Whether you’re working with standard or difficult-to-transfect cell lines, our reagents provide reliable, high-performance results every time.
Increase your AAV titer up to 10X with RevIT™ AAV Enhancer. RevIT is compatible with multiple serotypes, media, and HEK cell lines, making it an easy and effective addition to your workflow. By incorporating RevIT into your process, you can achieve significantly higher yields with minimal effort.
Utilize the most responsive support team in the industry. Our team of transfection experts is dedicated to helping you succeed. Our Field Application Scientists (FAS) provide on-site assistance to set up your research or manufacturing runs, saving you time and resources. Personalized support ensures that your processes are optimized from the start, reducing the risk of bioreactor issues or wasted materials.
Your drug assets require the support of GMP and ISO-compliant material, designed to support the commercial-scale production. Choose reagents and enhancers that maintain their results from the smallest shake flasks to the largest bioreactors. Through longer complex formulation times, your upstream processing team can more easily scale up to larger bioreactors with products designed to reduce risks and cut costs. Confidently move your AAV production from the lab to commercial manufacturing.

Join our Transfection Adoption Partnership (TAP) program to gain access to exclusive benefits, including:

  • Preferred pricing
  • Priority white glove service
  • Thought leadership opportunities

Partner AAV manufacturing companies receive tailored support to enhance their transfection processes, ensuring they remain at the forefront of AAV manufacturing innovation.

Ready to Speak to an Expert?

Explore Your Tools for Higher Titers

TransIT-VirusGEN Transfection Reagent

Set your lab up for AAV success with TransIT-VirusGEN™

RevIT AAV Enhancer

Improve your results by 2-4X with RevIT® AAV Enhancer

Rev Up Your AAV Genome Production

Take the first steps towards increasing your transfection efficiency by reading the free white paper on how the RevIT AAV Enhancer can be applied to your workflow. 

  • Read the free resource on how your lab can increase its AAV titer
  • Explore results across serotypes, media, and HEK cell lines
  • See data on how RevIT increases genomic titers without negatively impacting percent full capsids
  • Compare transfection reagents

How to Cut Costs:

2X the Number of Doses from a 200L Bioreactor

White Paper Preview:

See how TransIT-VirusGEN® + RevIT™ compare against other transfection reagents in both titer and percent full capsids.

VirusGEN® is the only transfection system to incorporate proprietary polymer and lipid technology – which enables both high efficiency and productivity.

VirusGEN offers roughly over a 6X increase in titer vs other transfection reagents

6.1X increase

in titer over Polymer P

VirusGEN offers roughly a 2X increase in percent full capsids vs other transfection reagents

~2X increase

in full capsids vs Polymer F

Work with a team of transfection experts to achieve upstream improvements, saving on bioreactor runs, capital commitments and more.

AAV Manufacturing Costs

Compare transfection reagents and cut your cost per dose in half. See how much your lab can save when scaling your viral production with the transfection calculator.

Labs and manufacturers save via:

  • Fewer bioreactor runs
  • Less pDNA required
  • Longer complex formulation windows
  • 8 other variables