Applications | Electroporation with Ingenio®

The Best Choice for High Efficiency Electroporation

Mirus Bio offers a full range of electroporation offerings, including; Ingenio® Electroporation Solution, electroporation cuvettes, and the new Ingenio® EZporator® Electroporation System. These products are designed to high efficiency delivery to hard-to-transfect cells without the cost or complication of other systems. Ingenio EZporator Electroporation Device and Reagent Product photo
  • High Efficiency Electroporation of Hard to Transfect Cells – Including primary cells and stem cells
  • Compatible with Most Conventional Electroporation Devices – Including the Ingenio® EZporator®, Lonza-Amaxa®, Bio-Rad® and Harvard BTX®
  • Cost Savings Using the Ingenio® Electroporation Kits – On your existing Amaxa® Nucleofector® without sacrificing performance
  • Long Shelf Life – One year shelf life allows maximum usage for your experiments
  • Buy Only What You Need – Available as solution alone or as part of a kit with cuvettes and cell droppers






Ingenio Electroporation Solution Cell Lines on Amaxa Data
Efficient Broad Spectrum Plasmid DNA Delivery Using Ingenio® Solution with Amaxa® Nucleofector® II. Ingenio® Electroporation Kits were used to transfect indicated cell types using Amaxa® Nucleofector® II Device. Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population. See the recommended experimental conditions (PDF).











Ingenio® Kits Provide a Cost-Effective, Stable Alternative to Nucleofector® Kitsα

Product Shelf Life Cost/Electroporationβ Savings
Amaxa® Nucleofector® Kit V (VCA-1003) 3 Months $15.20
Ingenio® Electroporation Kit (MIR 50112) 1 Year $9.16 40%
α Compatible with Amaxa® Nucleofector® II device βBased on 2016 U.S. list prices from company websites and protocol recommendations (25 RXN kit)


CRISPR RNP Electroporation of K562 Jurkat Cells Data
CRISPR RNP Delivery with Ingenio® Electroporation Solution. K562 and Jurkat cells were electroporated with a Cas9 protein/gRNA, ribonucleoprotein (RNP) complex, composed of 750 nM Cas9 protein (EnGen® Cas9 NLS, NEB) and 1500 nM pre-complexed two-part gRNA (IDT) targeting PPIB using the Ingenio® Electroporation Solution and a Gene Pulser Xcell™ Eukaryotic System. Exponential pulse conditions of 130V, 950 µF for K562 and 150V, 950 µF for Jurkat cells were applied to triplicate 0.2 cm cuvettes, 100 µl volume, 10 x 106 cells/ml +/- RNP complex. A T7E1 mismatch assay was used to measure cleavage efficiency

Same Results, Lower Cost Using Ingenio® with Amaxa® Nucleofector® II

 

Ingenio Electroporation Solution Similar Performance Amaxa Data

Ingenio® Solution Provides Comparable Efficiency on Amaxa® Nucleofector®. Cells were electroporated in parallel with an EGFP reporter vector using a Nucleofector® II Device (Amaxa®) and either the Ingenio® Electroporation Solution or the Nucleofector® Kit V (Amaxa®) according to Amaxa®’s recommended protocol for each cell line [HL-60 (T-019), Jurkat E6-1(X-001), K-562 (T-016), THP-1 (V-001), SK-N-MC (A-023), and MCF-7 (P020)]. EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population.

 

 

Ingenio Electroporation Solution Comparable Performance Amaxa BioRad Data

Ingenio® Solution Shows Similar Results with Amaxa® Nucleofector® and Other Electroporators. Ingenio® Solution used with Gene Pulser Electroporator Yields Comparable High Efficiency to the Amaxa® Nucleofector® II System: HL-60, Jurkat E6-1, K562, THP-1 and SK-N-MC cells were electroporated with the same EGFP reporter on the same day, using either Ingenio® Electroporation Solution in the Gene Pulser XCell (Bio-Rad®) (red bars) or the Amaxa® Solution V in Amaxa® Nucleofector® II (Lonza) (light grey bars).

 

High Electroporation Efficiency using Ingenio® Solution

 

Ingenio Electroporation Solution Toxicity and Efficiency vs PBS BioRad Data

Ingenio® Provides Both Superior Efficiency and Cell Viability Compared to Other Solutions. Cells were electroporated in parallel with an EGFP reporter vector using either Ingenio® Electroporation Solution, PBS or the Gene Pulser Electroporation Buffer (Bio-Rad®) on the Gene Pulser Xcell™ Eukaryotic System. (A) EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. (B) Cells were assayed for viablility by propidium iodide staining and flow cytometry analysis. Experiments were performed in triplicate on three separate days and the data averaged.

 

Ingenio® Kits provide a Cost-Effective and Stable Alternative to Nucleofector® Kitsa

ProductShelf LifeCost/ElectroporationbSavings
Amaxa® Nucleofector® Kit V (VCA-1003)3 months$14.64
Ingenio® Electroporation Kit (MIR 50112)1 year$8.6841%

a Compatible with Amaxa® Nucleofector® II device
b Based on 2014 U.S. List prices from company websites and protocol recommendations (25 RXN kit).

Ingenio® Solution Efficiently Delivers siRNA, miRNA and Viral RNA

 

Ingenio Electroporation Solution siRNA Data

Ingenio® Kits Deliver siRNA for Knockdown Using Different Electroporation Instruments. siRNA and plasmid DNA were co-electroporated with the indicated cell lines with the Ingenio® electroporation solution in 0.2 cm cuvettes using either the Gene Pulser (Bio-Rad®) or the Amaxa® Nucleofector® II (Lonza). 10µg/ml of plasmid encoding Firefly luciferase was co-electroporated with 250nM of either non-targeting siRNA control (Dharmacon Non-Targeting siRNA #1) or GL3 siRNA into Jurkat E6-1, HeLa and CHO-K1 cells. Twenty-four hours post electroporation, cells were harvested and assayed for luciferase activity. Data from independent experiments performed on different days were averaged then scaled to non-targeting siRNA control and are represented as a percentage of the control.

Pulse conditions used:

(1) Amaxa® Nucleofector® program:
a. Jurkat E6-1: X-001 with 10 x 106 cells/ml
b. HeLa: I-013 with 3 x 106 cells/ml
c. CHO-K1: U-023 with 5 x 106 cells/ml

(2) Bio-Rad® Gene Pulser Exponential Decay setting:
a. Jurkat: 150V, 950µF with 10 x 106 cells/ml
b. HeLa : 130V, 950µF with 3 x 106 cells/ml
c. CHO-K1: 150V, 900µF with 5 x 106 cells/ml

 

 

CRISPR RNP Electroporation of K562 Jurkat Cells Data

CRISPR RNP Delivery with Ingenio® Electroporation Solution. K562 and Jurkat cells were electroporated with a Cas9 protein/gRNA, ribonucleoprotein (RNP) complex, composed of 750 nM Cas9 protein (EnGen® Cas9 NLS, NEB) and 1500 nM pre-complexed two-part gRNA (IDT) targeting PPIB using the Ingenio® Electroporation Solution and a Gene Pulser Xcell™ Eukaryotic System. Exponential pulse conditions of 130V, 950 µF for K562 and 150V, 950 µF for Jurkat cells were applied to triplicate 0.2 cm cuvettes, 100 µl volume, 10 x 106 cells/ml +/- RNP complex. A T7E1 mismatch assay was used to measure cleavage efficiency at 48 hours post-transfection. Non-specific bands (NSP) were observed in the negative control of both cell lines. Cleavage efficiency was calculated based on the ratio of cleaved band intensities to the sum of cleaved and uncleaved band intensities minus the average signal of the non-specific band(s) in negative control lanes.

 

 

Ingenio Electroporation Solution iPS Cells Data

High Efficiency Plasmid DNA Electroporation of Human Induced Pluripotent Stem (iPS) Cells Using Ingenio®. The Ingenio® Electroporation Kit was used to transfect 2 x 106 iPS cells on the Amaxa® Nucleofector® II Device. Cells were electroporated with 8 µg ZsGreen expressing plasmid (Clontech) in 100 µl and plated in 6-well plates at 0.33 x 10cells/well. Cells were visualized 24 hours post-transfection and

Ingenio® EZporator® and Electroporation Solutions

Mirus Bio offers a universal solution for your existing electroporator as well as an innovative electroporation device

 

Ingenio Electroporation: A Universal Solution

Ingenio Electroporation Solution Sample

Ingenio® Electroporation Solution is compatible with multiple cell types and devices.

  • Use your existing system including the Ingenio® EZporator®, Nucleofector®, Bio-Rad® or Harvard BTX®
  • Reduce cost when using the Lonza Nucleofector® without sacrificing performance

 

Ingenio® EZporator Electroporation System

Ingenio EZporator Electroporation System

The Ingenio® EZporator® Electroporation System is an easy-to-use electroporation system for mammalian cells.

  • Easily optimize electroporation parameters for each cell type
  • Every system comes with a complimentary Ingenio® Electroporation Kit
 

Technical Resources

Don’t See Your Cell Type? Consult Reagent Agent® Transfection Database
Citation Database: Check if Ingenio® has been used by other researchers to transfect your cell type
Technical Support: Speak directly with a transfection expert

"The Ingenio® Electroporation Kit is routinely used in our lab to transfect induced pluripotent stem cells (iPSCs) via electroporation and yields extremely high transfection efficiency. The Ingenio® Kit is entirely compatible with the Amaxa® Nucleofector® and is also a much more cost effective solution."
Elizabeth Dominguez
Cellular Dynamics International (CDI)