In our lab we used
TransIT®-mRNA with EGFP mRNA as a control for transfection efficiency in the Sf9 insect cell line. Transfection efficiency was higher than 90%. (
Hera BioLabs)
Dr. Valeriya Steffey, M.D.
Director of In-Vitro Studies at Hera BioLabs
I used TransIT®-mRNA on Huh7.5.1 with mRNA to great effect. I saw lower toxicity with the TransIT®-mRNA as compared to other transfection reagents and much less toxicity than electroporation.
Beatrice Ary
UCSF Mission Bay
Our lab recently used the
TransIT-mRNA Transfection Kit to show that intracellular delivery of HPLC-purified and pseudouridine-containing mRNA can translate very efficiently without immune activation which is ideal for mRNA-based gene therapy applications.
TransIT®-mRNA further facilitated this work through low toxicity transfections of HEK 293T, human dendritic cells (DCs) and primary keratinocytes. (
Karikó et al. Nucleic Acids Research, 39:e142, 2011)
Dr. Katalin Kariko
University of Pennsylvania - Department of Neurosurgery