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Discovery Research

Spend less time searching for a transfection reagent and more time moving your innovations forward.

We offer a range of effective transfection solutions and tools for a comprehensive set of cell lines. These solutions are intentionally designed to be gentle on cells and maximize cell delivery while using a simplified, straightforward protocol.

Combine the Mirus Bio portfolio with our team of dedicated technical support scientists and you can get answers to compelling biological questions with less stress and more confidence.

Have a question? Let’s talk.

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Discovery Research Applications

CRISPR/Cas Genome Editing

Stem Cell Transfection

RNAi Gene Silencing

mRNA and Viral RNA Transfection

Stable Cell Line Generation

Low Toxicity Transfection

Co-transfection

Electroporation

TransIT-X2 Dynamic Delivery System Sample

TransIT-X2® Dynamic Delivery System

A novel, polymeric system for delivery of multiple nucleic acids to mammalian cells. Delivers CRISPR/Cas9 components in the following formats:

  • DNA – Deliver plasmid DNA expressing Cas9 or guide RNA
  • Guide RNA – Deliver guide RNA oligonucleotides targeting your gene of interest
  • RNP – Deliver Cas9/guide RNA ribonucleoprotein complexes

TransIT-mRNA Transfection Reagent Sample

TransIT®-mRNA Transfection Kit

A high efficiency, low toxicity transfection reagent for mammalian cells. Delivers CRISPR/Cas9 components in the following formats:

  • mRNA – Deliver messenger RNA expressing Cas9
  • Guide RNA – Deliver guide RNA targeting your gene of interest

Ingenio® Electroporation Solution

A high efficiency electroporation solution compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®. Delivers CRISPR/Cas9 components in the following formats:

  • DNA – Deliver plasmid DNA expressing Cas9 or guide RNA
  • mRNA – Deliver messenger RNA expressing Cas9
  • Guide RNA – Deliver guide RNA oligonucleotides targeting your gene of interest
  • RNP – Deliver Cas9/guide RNA ribonucleoprotein complexes

TransIT-X2 Transfection Reagent Sample

 

TransIT-X2® Dynamic Delivery System

An advanced system for delivery of CRISPR/Cas9 components in human IPSCs

  • Broad Spectrum Delivery – High efficiency delivery in a wide range of cell types
  • Genome Editing – Deliver Cas9 protein/guide RNA ribonucleoprotein (RNP) complexes
  • Technology – Novel, non-liposomal, polymeric delivery

 

TransIT-2020 Transfection Reagent Sample

 

TransIT®-2020 Transfection Reagent

A high performance, animal-free, broad spectrum DNA transfection reagent

  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
  • Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
  • Animal Origin Free – High performance with maximum compatibility

 

TransIT-LT1 Transfection Reagent Sample

 

TransIT®-LT1 Transfection Reagent

A low toxicity, broad spectrum DNA transfection reagent

  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • High Efficiency Delivery – Achieve expression in a large population of cells

 

TransIT-mRNA Transfection Reagent Sample

TransIT®-mRNA Transfection Kit

A high efficiency, low toxicity transfection reagent for large RNA delivery

  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • High Efficiency Delivery – Achieve RNA delivery in a large population of cells
  • Serum Compatible – Perform transfections in the presence of serum which eliminates the need for a media change

 

Ingenio Electroporation Solution Sample

Ingenio® Electroporation Solution

High efficiency electroporation using any instrument

  • High Efficiency Electroporation – Deliver to hard to transfect cell lines and primary cells
  • Compatible with Most Conventional Electroporation Devices – Use your existing system including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®
  • Save Money Without Sacrificing Performance – Available as a stand-alone solution or as part of a complete kit with cuvettes and cell droppers

Broad Spectrum TransIT® Transfection Reagent Recommendations for Gene Silencing

Nucleic Acid to be TransfectedTransfection Reagent Recommendation
siRNA/miRNA

Option 1: TransIT-X2® Dynamic Delivery System

Option 2: TransIT-TKO® Transfection Reagent

Option 3: TransIT-siQUEST® Transfection Reagent

shRNA encoding Plasmid DNA libraries

Option 1: TransIT-X2® Dynamic Delivery System

Option 2: TransIT®-2020 Transfection Reagent

Option 3: TransIT®-LT1 Transfection Reagent

siRNA/miRNA and Plasmid DNATransIT-X2® Dynamic Delivery System

TransIT-mRNA Transfection Sample

TransIT®-mRNA Transfection Kit

Each Kit is supplied with the TransIT®-mRNA Transfection Reagent and the mRNA Boost Reagent

  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • High Efficiency Delivery – Achieve RNA delivery in a large population of cells to ensure experimental success
  • Serum Compatible – Perform transfections in the presence of serum which eliminates the need for a media change and maintains cellular health
  • Deliver Various Sizes of RNA – Ideal for specialized applications, such as virus production, protein expression and CRISPR genome editing

TransIT-X2 Transfection Reagent Sample

TransIT-X2® Dynamic Delivery System

A high efficiency, low toxicity transfection reagent for large RNA delivery

  • Efficiency –Exceptional broad spectrum transfection
  • Versatility –Cutting edge delivery of plasmid DNA and small RNAs
  • Technology – Novel, non-liposomal, polymeric delivery

 

TransIT-LT1 Transfection Reagent Sample

TransIT®-LT1 Transfection Reagent

A low toxicity, broad spectrum DNA transfection reagent

  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
  • Low Cellular Toxicity – Maintain cell density and reduce experimental biases
  • High Efficiency Delivery – Achieve expression in a large population of cells

 

TransIT-2020 Transfection Reagent Sample Size

TransIT®-2020 Transfection Reagent

A high performance, animal-free, broad spectrum DNA transfection reagent

  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines
  • Gentle to Your Cells – Balances high efficiency nucleic acid deliver and low cellular toxicity
  • Animal Origin Free – High performance with maximum compatibility

TransIT®-LT1 Transfection Reagent

  • Broad Spectrum DNA Delivery – Utilize one transfection reagent and protocol for a variety of cells
  • High Efficiency Delivery – Achieve expression in a large population of cells for experimental success
  • Deliver Single or Multiple Plasmids – Suitable for applications such as virus production

 

TransIT®-2020 Transfection Reagent

  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines and primary cells
  • Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity
  • Animal Origin Free – High performance with maximum compatibility

Different TransIT® Transfection Reagents are recommended depending on the combinations of nucleic acids being delivered. All of the following reagent recommendations are of broad spectrum formulations that are chemically distinct. Depending on the cell type being transfected, one reagent may have superior performance over others; for cell-type specific recommendations, please consult the Reagent Agent® transfection database or contact techsupport@mirusbio.com

 

Nucleic Acid(s) to be Co-transfectedTransfection Reagent
Multiple plasmid DNAsOption 1: TransIT-X2® Dynamic Delivery System
Option 2: TransIT®-2020 Transfection Reagent
Option 3: TransIT®-LT1 Transfection Reagent
Multiple siRNAsOption 1: TransIT-X2® Dynamic Delivery System
Option 2: TransIT-TKO® Transfection Reagent
Option 3: TransIT-siQUEST® Transfection Reagent
Plasmid DNA and siRNATransIT-X2® Dynamic Delivery System
Multiple mRNAsTransIT®-mRNA Transfection Kit
Plasmid DNA and mRNATransIT-X2® Dynamic Delivery System and
TransIT®-mRNA Transfection Kit
Multiple oligonucleotidesTransIT®-Oligo Transfection Reagent
Plasmid DNA and oligonucleotidesTransIT-X2® Dynamic Delivery System and
TransIT®-Oligo Transfection Reagent
Other combinationsContact techsupport@mirusbio.com
 
Ingenio Electroporation Solution Sample

Ingenio Electroporation: A Universal Solution

Ingenio® Electroporation Solution is compatible with multiple cell types and devices.

  • Use your existing system including the Ingenio® EZporator®, Nucleofector®, Bio-Rad® or Harvard BTX®
  • Reduce cost when using the Lonza Nucleofector® without sacrificing performance

Ingenio EZporator Electroporation System

Ingenio® EZporator Electroporation System

The Ingenio® EZporator® Electroporation System is an easy-to-use electroporation system for mammalian cells.

  • Easily optimize electroporation parameters for each cell type
  • Every system comes with a complimentary Ingenio® Electroporation Kit