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TransIT®-2020 Transfection Reagent

A high performance, animal-free, broad spectrum DNA transfection reagent

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  • Broad Spectrum DNA Delivery – Achieve high expression in many cell types, including hard to transfect cell lines and primary cells
  • Gentle to Your Cells – Balances high efficiency nucleic acid delivery and low cellular toxicity  Learn more>>
  • Animal Origin Free – High performance with maximum compatibility

Figures and Data

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TransIT-2020 Reagent Exhibits Higher Expression and Lower Cell Toxicity Compared to Other Transfection Reagents

TransIT®-2020 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents. Human umbilical vein endothelial cells (HUVEC) were transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph. Error bars represent the standard deviation of triplicate wells.

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High Performance Plasmid Transfection of Primary Cells

High Performance Plasmid Transfection. Primary Human Small Epithelial Cells (HSAEpic) were transfected using TransIT®-2020 and an EGFP expression plasmid (4:1 reagent-to-DNA ratio). Images were taken 24 hours post-transfection using a Zeiss axiovert inverted fluorescence microscope.

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Superior Gene Expression in a Broad Spectrum of Cell Types

Superior Gene Expression in a Broad Spectrum of Cell Types. The indicated cell types were transfected in 96-well plates with a luciferase expression plasmid (0.1 µg/well) according to industry accepted testing protocols. Reagent to DNA ratios were optimized for each cell type: TransIT®-2020 (Mirus Bio, 2:1 or 3:1), FuGENE® HD (Roche, 3.5:1), Lipofectamine® 2000 (Life Technologies, 1.5:1, 3:1 or 5:1).  Luciferase activity was measured 24 hours post-transfection. Values were normalized to TransIT®-2020 and presented as a percentage of luciferase expression. FuGENE® is a registered trademark of Fugent LLC. Lipofectamine® is a trademark of Life Technologies Corporation.

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Specifications

Storage Conditions:
All Configurations: Store at -20°C

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


Additional Resources

COA Lookup

Download Certificates of Analysis

COO Lookup

Download Certificate of Origin

CRISPR Experimental Data
Preliminary Data: Western blot from non-clonal cell populations demonstrating efficient targeting of three genes of interest.

Testimonials

I recently tested TransIT®-2020 and TransIT®-LT1, and both reagents worked well in terms of their efficiency at transfecting human-derived iPS cells with CRISPR constructs and a fluorescent protein reporter. Through visual inspection, transfection efficiencies with TransIT®-2020 and TransIT®-LT1 were clearly higher than with Lipofectamine® 3000.

Our lab routinely works with primary human fibroblasts which are notoriously difficult to transfect, and we require a method that is non-toxic, reliable, and reproducible. TransIT®-2020 is the only reagent on the market that fits those criteria. In addition, TransIT®-2020 also transfects colon cancer cell lines HCT-116 and DLD1 with much higher efficiencies. Overall, the pace and quality of our research have improved since we started using TransIT®-2020.

...We have been able to transfect BMDM’s with the TransIT®-2020 Transfection Reagent. Not only are my cells healthier, but I get good expression, and it is more cost effective to the Neon Transfection System that my lab traditionally uses. I have also been able to share these results with others that are now trying it with great success. It makes me look like a hero!

...However, I did find a gem: The TransIT®-2020 reagent from Mirus Bio LLC. I tested five or six breast cancer cell lines, and in general, my transfection efficiency was 5-10 fold higher with the TransIT®-2020reagent than with the "standards" - Lipofectamine®...with essentially no cell death. I also saw, for the first time ever, true 100% transfection efficiency of HEK293 cells - every last cell on the plate, glowing bright GFP-green.

TransIT®-2020 Transfection Reagent has been working well in our CRISPR genome editing experiments in HEK 293 cells. I appreciate the fact that the transfection protocol is straightforward, the toxicity appears to be low and we obtained high CRISPR knockout efficiency.

I was using Lipofectamine® to transfect HEK cells and was only getting about 20% transfection rates but after using TransIT®-2020 it went from 20% to 70%! I highly recommend using this reagent. It was very easy to follow the protocol and I obtained high cell viability. (Review published in SelectScience)