TransIT®-2020 transfection reagent is easy to use and gives better transfection efficiency for MSC transfection compared to Lipofectamine® 3000.
Li Xu
Duke University
I recently tested TransIT®-2020 and TransIT®-LT1, and both reagents worked well in terms of their efficiency at transfecting human-derived iPS cells with CRISPR constructs and a fluorescent protein reporter. Through visual inspection, transfection efficiencies with TransIT®-2020 and TransIT®-LT1 were clearly higher than with Lipofectamine® 3000.
Fedir Kiskin
University of Cambridge
Our lab routinely works with primary human fibroblasts which are notoriously difficult to transfect, and we require a method that is non-toxic, reliable, and reproducible. TransIT®-2020 is the only reagent on the market that fits those criteria. In addition, TransIT®-2020 also transfects colon cancer cell lines HCT-116 and DLD1 with much higher efficiencies. Overall, the pace and quality of our research have improved since we started using TransIT®-2020.
John A. Burns
David Scicchitano Lab, New York University
TransIT®-2020 Transfection Reagent has been working well in our CRISPR genome editing experiments in HEK 293 cells. I appreciate the fact that the transfection protocol is straightforward, the toxicity appears to be low and we obtained high CRISPR knockout efficiency.
Steve Plouffe
Sanford Consortium for Regenerative Medicine
...However, I did find a gem: The TransIT®-2020 reagent from Mirus Bio LLC. I tested five or six breast cancer cell lines, and in general, my transfection efficiency was 5-10 fold higher with the TransIT®-2020 reagent than with the "standards" - Lipofectamine®...with essentially no cell death. I also saw, for the first time ever, true 100% transfection efficiency of HEK293 cells - every last cell on the plate, glowing bright GFP-green.
Nicole Perry
Kontrogianni Lab, University of Maryland
...We have been able to transfect BMDM’s with the TransIT®-2020 Transfection Reagent. Not only are my cells healthier, but I get good expression, and it is more cost effective to the Neon Transfection System that my lab traditionally uses. I have also been able to share these results with others that are now trying it with great success. It makes me look like a hero!
Jeffrey Fay
Hoffman Lab, University of Hawaii at Manoa
I was using Lipofectamine® to transfect HEK cells and was only getting about 20% transfection rates but after using
TransIT®-2020 it went from 20% to 70%! I highly recommend using this reagent. It was very easy to follow the protocol and I obtained high cell viability. (
Review published in SelectScience)
Dr. Olivia George
Univeristy of New Mexico - Department of Molecular Genetics and Microbiology
We observed transient transfection of a tricky protein (huge, possibly toxic 250 kDa protein) for the first time. The 2020 reagent enabled this where the TransIT®-293 and TransIT®-LT1 did not. Great product!
Charlie Fehl
Wayne State University
Using TransIT®-2020, we transfected HeLa cells in 6-well plates with 1.25 µg of the Zheng lab construct (pX330) from Addgene that harbors both a specific guide RNA against a recognition sequence in our gene of choice, and 1.25 µg of a donor plasmid with 1 kb of 5’ and 3’ homology sequence. We then selected the cells using puromycin and came across a population that harbored the modification we were interested in. Thank you so much for the sample of TransIT®-2020. Mirus has always been without exception the gold standard for me and why anyone else would want to use anything else is just beyond me.
Aviva Joseph
University of Massachusetts Medical School