Our lab is interested in identifying barriers to influenza virus cross-species transmission and how those control the emergence of new pandemic viruses... we were able to use
TransIT®-LT1 in our studies to further implicate the viral polymerase complex as a major regulator of viral tropism and identify new adaptive strategies used by influenza as it moves from birds to people. Details of these efforts can be found in the Journal of Virology. (
Mehle, A et al. Journal of Virology. Feb 2012)
Dr. Andrew Mehle
University of Wisconsin - Madison
Our lab tested TransIT®-LT1 from Mirus for virus production in 293T cells. At a 3:1 ratio using up to 5 µg of lentivirus, packaging and envelope plasmid, TransIT®-LT1 was far superior to Lipofectamine® in terms of viral titer downstream. So much so that our lab purchased the 0.4 ml product after using the trial.
Andrew Paluch
University of Cincinnati
We routinely use Mirus
TransIT®-LT1 Transfection Reagent for the delivery of plasmid DNA to carry out immunoprecipitation experiments. Our lab recently published using
TransIT®-LT1 for this application to reveal a crucial regulator (MCUR1) for calcium uptake in the mitochondria to regulate cellular metabolism. (
Mallilankaraman, K et al. Nature Cell Biology. December 2012)
Dr. Karthik Mallilankaraman
Madesh Lab, Temple University
I recently tested TransIT®-2020 and TransIT®-LT1, and both reagents worked well in terms of their efficiency at transfecting human-derived iPS cells with CRISPR constructs and a fluorescent protein reporter. Through visual inspection, transfection efficiencies with TransIT®-2020 and TransIT®-LT1 were clearly higher than with Lipofectamine® 3000.
Fedir Kiskin
University of Cambridge
Our lab regularly transfects head and neck cancer squamous carcinoma cell lines as well as pre-cancerous oral lesion cell lines and primary cells with Mirus Bio products. We recently published a paper using Mirus Bio
TransIT®-LT1 in which we concluded tobacco carcinogens up-regulate AP-1 activity and AP-1 dependent IL-8 and VEGF gene expression in head and neck cancer. (
Swenson, W et al. Molecular Carcinogenesis. April 2011)
Beverly Wuertz
Ondrey Lab, University of Minnesota