Clinic to Commercial Manufacturing: Higher Titers and Product Quality with TransIT® AAViator GMP
Today’s TransMission is a SNiP of one of our favorite papers featuring Label IT®. Nasri and Mir et al. describe an elegant use for Label IT® in enriching CRISPR/Cas-edited cells. For use in the clinic, it is highly desirable to limit the number of nonedited cells that may compete with the therapeutic, modified cells. Read on to learn how Label IT® was used to enhance the CRISPR genome-editing workflow!
Today’s TransMission explores an unconventional application of Mirus Bio’s Label IT® Nucleic Acid Labeling kits in engineering a new method of vaccine delivery, the microneedle array (MNA). MNAs are patches of micron-scale needles that delicately penetrate the skin in a painless manner, accessing an immune-rich population of cells. In this study, the authors fluorescently labeled immune-stimulating oligos using Label IT® Cy®3 and Label IT® Cy®5 to visualize their distribution on MNAs.
Read on to learn more about this unique method of stimulation!
Ideal labeling density will depend on the nucleic acid being labeled and the intended downstream application of the labeled nucleic acid. With Label IT® nucleic acid labeling technology, the labeling density can easily be adjusted. In this Tip from the Bench, we discuss two methods for tweaking the labeling density.
The labeling density for nucleic acids labeled with fluorescent Label IT® dyes can be calculated easily using spectrophotometry. Read this Tip from the Bench to learn how to calculate the base:dye ratio and the pmol of dye/µg nucleic acid.
Label IT® reagents are available in several formats, which provides researchers with a variety of options for their nucleic acid labeling experiments. In this Tip from the Bench, we cover the difference between the four formats of Label IT® kits and provide guidance on which to choose for a given application.
Today’s TransMission is a SNiP (Small News in Pieces). Read how Jack et al. use TransIT® and Label IT® to study telomeres.