Clinic to Commercial Manufacturing: Higher Titers and Product Quality with TransIT® AAViator GMP
VirusGEN Stabilizer 15mL

VirusGEN® Transfection Complex Stabilizer – 15 mL (for 50 L of cell culture)

The VirusGEN Stabilizer combined with TransIT-VirusGEN supports the formation of transfection complexes that exhibit minimal growth from 30 minutes to 3 hours, eliminating the need for later stabilization steps to deliver consistent performance (titer, percent full capsids).

SKU MIR 6775 Categories , , Tags ,
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Description

The VirusGEN® Transfection Complex Stabilizer is an additive used with TransIT-VirusGEN® Transfection Reagent and RevIT™ AAV Enhancer to reduce the transfection complex volume from 5% to 2% and stabilize the transfection complex for up to three hours without sacrificing performance.

  • Up to three-hour stability window removes the stress of precise transfection timing
  • Lower transfection complex volume requirement minimizes waste and operational complexity
  • Optimal yield at any scale with consistently high titers and full capsid percentage

Supporting Data

Scale AAV Production with Peace of Mind

Extend transfection complex formation time by up to 3 hours, reduce complex volume by >50%, and maintain high titers and full capsids.

Extend your transfection complex delivery window out to 3 hours with high titers and percent full capsids while using just 2% culture volume.
The VirusGEN® Transfection Complex Stabilizer is an additive used with TransIT-VirusGEN® Transfection Reagent and RevIT™ AAV Enhancer to reduce the complex volume to 2% and stabilize the transfection complex for 3 hours without sacrificing performance.

Scale with Confidence: With VirusGEN Transfection Complex Stabilizer, manufacturers can simplify scaling to larger bioreactors.

Chart showing sustained AAV productivity up to 180 minutes with VirusGEN + VirusGEN Stabilizer + RevIT with only. Polymer F's productivity sinks by the 30 minute mark.

Precision Through Stability: Transfection complexes grow as cationic polymers, cationic lipids and DNA interact, resulting in an optional time and size window for addition of the transfection complexes. This can create challenges at large scale. With VirusGEN Stabilizer:

  • Complex growth is inhibited, keeping particles within the ideal size range for efficient transfection
  • Higher titers and full capsid percentages are maintained over extended time periods
  • Decrease transfection complex volume to simplify transfection complex addition to large-scale bioreactors

Chart showing high titer and small transfection complex radius are sustained for 120 minutes with just 2% culture volume. Vs 5% culture volume where titer decreases in the 120 minute window because of the increase in transfection complex radius.

A stabilized transfection complex stays under 1000 nm. Contrasted with an unstabilized transfection complex which will grow past a point of viability.

Resources

Research Poster

Stability Meets Scalability
2026, Jim Ludtke et. al.

Specifications

Storage Conditions
All Configurations: Store at -10 to -30ºC

Product Guarantee
All Configurations: 3 months

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

Technical Product Literature

Full Protocols

VirusGEN® AAV Kit with RevIT™ AAV Enhancer and VirusGEN® Stabilizer Protocol (PDF)

SDS
VirusGEN® Stabilizer SDS (PDF)

For a lot-specific Certificate of Analysis (COA) or Certificate of Origin (COO) for this product, please contact techsupport@mirusbio.com.

FAQs

See the VirusGEN AAV FAQs

Using transfection reagents and enhancers from Mirus has given our platform a competitive advantage. Increasing efficiency and lowering costs for all of our programs.

Sally Mader, PhD
ZYZ Theraputics