Clinic to Commercial Manufacturing: Higher Titers and Product Quality with TransIT® AAViator GMP

VirusGEN® AAV Kit + RevIT™ for 10L Culture – With VirusGEN® Stabilizer

Proven Nucleic Acid Delivery for Large-Scale Virus Production | Now includes the VirusGEN Transfection Complex Stabilizer

Description

The VirusGEN® AAV Transfection Kit with RevIT™ AAV Enhancer and VirusGEN® Stabilizer is the first and only transfection kit designed to simplify upstream AAV scale-up. The kit includes: 

  • TransIT-VirusGEN® Transfection Reagent proven for AAV production in suspension cells.
  • RevIT™ AAV Enhancer designed to increase AAV titer 2-4X for any serotype, reagent, HEK293 cell line or media.
  • VirusGEN® Transfection Complex Stabilizer, which extends transfection complex formation time by up to 3 hours and reduces transfection complex volume down to 2% of total culture volume, while maintaining high titers and percent full AAV capsids.

SKU: MIR 8008

Supporting Data

 

 

 

RevIT™ AAV titer enhancer improves titer across serotypes when used with polymer-only transfection reagents.

 

 

 

RevIT™ AAV titer enhancer can be used with any media or cell line to increase titer.

 

 

VirusGEN® plus RevIT™ AAV titer enhancer outperformed polymer-only reagents.

 

RevIT™ AAV titer enhancer improves titer across serotypes when used with the VirusGEN® transfection platform.

VirusGEN® Stabilizer extends transfection complex formation time by up to 3 hours and reduces transfection complex volume down to 2% of total culture volume.

Resources

Specifications

Storage Conditions
All Configurations: Store at -20ºC, protected from exposure to light.

Product Guarantee
All Configurations: 6 months

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

 

Technical Product Literature

Full Protocols
VirusGEN® AAV Kit with RevIT™ AAV Enhancer Protocol (PDF)
VirusGEN® AAV Kit with RevIT™ AAV Enhancer and VirusGEN® Stabilizer Protocol (PDF)

Quick Reference Protocol
RevIT™ AAV Enhancer QRP (PDF)
VirusGEN® AAV Kit with RevIT™ AAV Enhancer QRP (PDF)

SDS
RevIT™ AAV Enhancer SDS (PDF)
VirusGEN® AAV Kit with RevIT™ AAV Enhancer SDS (PDF)
VirusGEN® Stabilizer SDS (PDF)

Additional Resources

Virus Titer Reference Card: Methods to Measure Lentivirus and Adeno-associated Virus in Your Sample (PDF)

For a lot-specific Certificate of Analysis (COA) or Certificate of Origin (COO) containing a TSE/BSE Statement for this product, please contact techsupport@mirusbio.com.

FAQs

See the VirusGEN AAV FAQs

Using transfection reagents and enhancers from Mirus has given our platform a competitive advantage. Increasing efficiency and lowering costs for all of our programs.

Sally Mader, PhD
ZYZ Theraputics