I have been using the Ingenio® Electroporation Solution to generate knockouts using the CRISPR/Cas system in primary neural stem cells. The transfection efficiency is comparable to the Amaxa® kit, and knockout generation is quite efficient using wt-Cas9 plasmids. Multiple gRNAs have yielded comparable efficiency.
Simon Weissman
Biotech Research and Innovation Centre, Denmark
I like the Ingenio® Electroporation Solution a lot. It is simple, efficient and reasonably priced. Also, I like that you can use it with multiple different instruments. The Ingenio® EZporator® Electroporation system worked great for our lab too. I love that you have some control over the electroporation settings, but its not too complicated.
Artem Nemudryi
Montana State University
I was very depressed for the last 6 months because I was unable to transfect my rat cell line with various transfection reagents. I tried 5 Nucleofection® programs, 2 buffers and several different cell densities. But nothing worked. I am very happy to inform you, Ingenio® is a life saver!
Sanal Madhusudana Girija
Albert Einstein College of Medicine
The Ingenio® Electroporation Kit is routinely used in our lab to transfect induced pluripotent stem cells (iPSCs) via electroporation and yields extremely high transfection efficiency. The Ingenio® Kit is entirely compatible with the Amaxa® Nucleofector® and is also a much more cost effective solution.
Elizabeth Dominguez
Cellular Dynamics International (CDI)