TransIT^®-Oligo Transfection Reagent – 1.0 mL

Description

• Unique Formulation – Maximize transfection performance of oligonucleotides into a wide range of cells
• Low Cellular Toxicity – Maintain cell density and reduce experimental biases
• High Efficiency Delivery – Achieve high transfection efficiency in cells to ensure experimental success

Oligonucleotides tested:
phosphodiester DNA, phosphothioate DNA (sDNA), phosphothioate RNA (sRNA), 2’OMe RNA, 2’OMe RNA/sDNA Chimerics, Morpholino/DNA duplexes, siRNA and miRNA.

Mirus recommends TriLink BioTechnologies, Inc. for a variety of modified and custom oligonucleotides. Learn more by clicking on their logo below.

TransIT®-Oligo Reagent Achieves High Transfection Efficiency. HeLa cells transfected using TransIT®-Oligo Reagent and Label IT® Cy®3 and Label IT® Fluorescein labeled phosphothioate DNA oligos in complete media for 24 hours.

TransIT®-Oligo Reagent Effectively Transfects a 2’OMe RNA Oligo that Blocks a Cryptic Splice Site. The HeLa-Luc 705 reporter cell line (Kang et al. 1998, 37:6235) used in this study contains a luciferase reporter construct that has the ß-globin 705 intron inserted into the luciferase ORF. A mutation present at position 705 of the ß-globin intron activates two cryptic splice sites within the intron that lead to the production of a spliced luciferase mRNA that is disrupted by a small intron with an in-frame stop codon, thus preventing translation of functional luciferase protein. The transfection of a 2’OMe oligonucleotide (TriLink BioTechnologies, Inc.) complementary to the cryptic 705 splice site inhibits splicing at the cryptic splice sites enabling the complete removal of the ß-globin intron and production of a mRNA with a complete, uninterrupted luciferase ORF.

The HeLa-Luc 705 cell line was transfected with increasing amounts of the anti-705 splice site 2’OMe RNA oligo at the indicated final concentrations using the TransIT®-Oligo Transfection Reagent. The cells were harvested 24 hours post-transfection and assayed for luciferase activity. The increase in luciferase activity indicates effective delivery of the anti-705 splice site RNA oligo using the TransIT®-Oligo Reagent.

SKU: MIR 2160