Mirus Bio receives ISO 13485:2016 certification, underscoring the quality of processes used to support GMP product portfolio. Read more
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Mirus Bio receives ISO 13485:2016 certification, underscoring the quality of processes used to support GMP product portfolio. Read more
Frequently Asked Questions | In Vivo Delivery REQUEST AN EVALUATION In Vivo Delivery pLIVE® In Vivo Vectors The pLIVE® Vector (Liver In Vivo Expression) is designed for high level, prolonged
Applications | RNAi Gene Silencing TransIT for Gene Silencing siRNA Mediated Pathway miRNA Mediated Pathway shRNA Mediated Pathway Products TransIT® Transfection Reagents for Gene Silencing
Because siRNA differs in size and structure from plasmid DNA, transfection reagents can be optimized and formulated differently for delivery of these RNA molecules. After selection of the appropriate transfection reagent, optimization of several experimental parameters is key to achieving the highest efficiency siRNA transfection and corresponding effective knockdown of target gene expression.
Here we highlight our top “Tips from the Bench” for optimizing siRNA delivery. Note: most of these tips are applicable to delivery of other classes of oligos, including but not limited to miRNA and antisense oligonucleotides.
Frequently Asked Questions | Transfection TransIT-LT1 TransIT-X2 TransIT-2020 TransIT-mRNA TransIT-PRO TransIT-siQUEST TransIT-TKO TransIT®-LT1 Transfection Reagent TransIT®-LT1 Transfection Reagent is a broad spectrum, low toxicity transfection
The labeling density for nucleic acids labeled with fluorescent Label IT® dyes can be calculated easily using spectrophotometry. Read this Tip from the Bench to learn how to calculate the base:dye ratio and the pmol of dye/µg nucleic acid.
Applications | High Throughput Transfection High Throughput Reverse Transfection of DNA RNAi Screening Protocol HT Citations and Reagents Products High Throughput Transfection Advances in high