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Pairing a novel enhancer with transfection reagents to enable high-titer rAAV production

On Demand Webinar

With the success of cell and gene therapies and exponentially increasing demand, the second wave of viral vector manufacturing is focused on large-scale suspension cell culture. Upstream manufacturing processes for recombinant adeno-associated virus (AAV) and lentivirus (LV) are largely dependent on transient transfection of multiple plasmid DNA constructs encoding essential viral proteins. To produce the virus needed for these therapies, processes must be adapted to bioreactor scale for cGMP production. In response to this need, Mirus Bio has established solutions including a novel transfection reagent that increases AAV and LV titers 2-10-fold over existing technologies, and a unique enhancer that increases titer over the reagent up to 4X.

This webinar adopts a roundtable format with a panel comprising end users as well as a Mirus Bio senior research scientist. 

Watch this webinar to:

  • Understand how you can achieve your viral vector process productivity milestones
  • Find out how the VirusGEN® platform increases production across multiple transfection workflows and a variety of AAV serotypes
  • Enable reductions in cost per dose for cell and gene therapies
  • Streamline your journey from a technical evaluation through implementation

The expert panel

Florian Dziopa

Director of Upstream Process Development, MeiraGTx

Florian holds a B.Eng degree in chemical engineering from Polytechnique Montreal and a Doctorate in Pharmacy from University of Lyon. He has 9 years of experience in Upstream Process Development and worked on optimizing several process modalities, ranging from E.Coli fermentation for antibody fragment production to adherent and suspension mammalian cell culture for AAV manufacturing. Currently, he is the Director of Upstream Process Development at MeiraGTx, where he spent the last 6 years developing and optimizing AAV manufacturing platforms based on suspension HEK293 cells and transient transfection.

René Gantier

Senior Director R&D, Advanced Bioprocess Applications, Repligen


René Gantier is the Senior Director R&D, Advanced Bioprocess Applications at Repligen. He has more than 20 years of experience developing production and purification processes for biotherapies. René holds a MSc in Cell Biology from the University of Nantes (France) and a PhD in Biochemistry from the University of Rouen (France). He started his career in the biotechnology industry as a scientist developing long-lasting therapeutic cytokines at Nautilus Biotech (France), before joining Pall Life Sciences as a downstream processing field application specialist, and then expanded his role to lead the Biotech Process R&D team developing end-to-end upstream-downstream continuous bioprocessing solutions for mAbs and cell/gene therapies. In his current role at Repligen, he leads the development of advanced bioprocess applications and technologies with a focus on next generation cell and gene therapy manufacturing processes.

Becky Reese

R&D Senior Scientist I, Mirus Bio
 
Dr Becky Reese is a Senior Scientist in Research and Development at Mirus Bio LLC, a company committed to equipping biopharmaceutical innovators with next generation transfection solutions for all cell culture applications. Becky has over 10 years of experience in cellular and molecular biology and has worked with a wide array of cell lines, transfection technologies, and molecular assays. Becky has a PhD in Cellular and Molecular Biology from the University of Wisconsin – Madison and has developed a key interest in making the viral vector manufacturing efficient, scalable, and user-friendly.