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Mirus Bio Transfection Solutions

Our focus: Your solution.

Every transfection product offered by Mirus Bio has been developed by an in-house team of organic chemists, molecular biologists, cell biologists, and biochemists. Mirus Bio scientists continue to develop state-of-the-art, nonviral, self-assembling complexes (transfection complexes) that serve as DNA or RNA carriers. These complexes are a mixture of a nucleic acid (DNA or RNA) with the carefully selected, appropriate transfection formulation to provide highly efficient and reproducible delivery into a wide variety of cells. The delivery mechanism mimics virus delivery in several key ways, including active transport into the cell (endocytosis) and endosomal destabilization and release.

All Mirus Bio products are developed and manufactured in our Madison, Wisconsin laboratories.

Lipid and Polymer Transfection of DNA

Mirus Bio offers a very comprehensive, selection of non-liposomal TransIT® Transfection Reagents. These reagents are based on blends of polymers and/or lipids developed for DNA transfection with minimal toxicity and are all compatible with serum-containing media. TransIT® Transfection Reagents have been developed for the following applications: transfection of both DNA and/or siRNA in multiple cell types; broad-spectrum delivery into a wide variety of cells; and increased protein production. The TransIT® product line also includes a panel of cell type-specific DNA delivery reagents.

TransIT-X2® for Transfection of DNA and siRNA into Multiple Cell Types

For optimal delivery of either DNA or siRNA into a variety of cell types, Mirus offers the TransIT-X2® Dynamic Delivery System. This novel formulation relies on a nonliposomal, polymeric chemistry system for efficient delivery of nucleic acids through cellular endosomal processes. The TransIT-X2® Dynamic Delivery System provides multiple advantages over outdated liposomal methods, including better DNA delivery, more efficient delivery of siRNA, and less toxicity across multiple cell types.

TransIT® Reagent for Broad-spectrum DNA Delivery

Mirus offers two reagents, TransIT®-LT1 and TransIT®-2020, for plasmid DNA delivery into a wide variety of cell types. TransIT®-LT1 has been Mirus Bio's flagship reagent since 1996 and offers a Low Toxicity solution for all nucleic acid delivery needs. More recently, TransIT®-2020 was developed for increased performance across a multitude of cell types including hard-to-transfect cell types such as primary and stem cells.

For high-throughput applications involving DNA transfection, TransIT®-Express provides a broad-spectrum reagent optimized for 96-well plate applications. This reagent provides the same low cellular toxicity and high efficiency as other TransIT® reagents.

TransIT® Reagent for Protein Production

Protein Production

The Transfection Experts at Mirus developed the TransIT-PRO® Transfection Kit to address the need to increase protein production for use in biotherapeutics. The TransIT-PRO® Transfection Kit consists of a DNA transfection reagent and optional boost reagent combination specifically developed for mammalian protein production in suspension HEK 293- and CHO-derived cells. The PRO Boost Reagent enhances expression in certain media formulations. TransIT-PRO® Transfection Reagent and PRO Boost Reagent are comprised of animal origin-free components that are compatible with many chemically defined media formulations. The TransIT-PRO® Transfection Kit eliminates the need for a culture medium change after transfection and is suitable for both transient and stable transfections.

TransIT® Reagent for Cell Line–Specific Transfection

Insect GFP

With almost two decades of gene delivery experience, The Transfection Experts at Mirus have amassed a large collection of cell type-specific reagents with all of the same low-toxicity and high-performance qualities of the broad-spectrum reagents. These reagents are suitable for transfection of the most common cell types:

Additionally, a variety of other reagents are available to address groups of commonly used but hard-to-transfect cell lines, including:

Lipid and Polymer Transfection of RNA and Oligos

Mirus Bio also offers TransIT® Transfection Reagents for RNA and oligonucleotide (oligo) delivery into any cell type. These reagents address delivery of large RNA, siRNA/miRNA, and short oligos with minimal toxicity and are all compatible with serum-containing media. These reagents are based on blends of polymers and/or lipids suited for broad-spectrum delivery applications.

TransIT® Reagent for Large RNA (e.g., mRNA and viral RNA)

The TransIT®-mRNA Transfection Kit delivers large RNA directly to the cytoplasm for expression, which avoids transcriptional regulation effects. The TransIT®-mRNA Transfection Kit can be used to deliver a variety of RNA molecules, including mRNAs and viral RNAs (2–10 kb). This kit can be used for multiple applications, such as short-term protein expression, viral production, and replication studies.

The TransIT®-mRNA Transfection Kit contains two components: TransIT®-mRNA Reagent and the mRNA Boost Reagent. This kit is compatible with serum-containing media; transfection efficiency is optimal when transfections are performed in the presence of serum, with no medium change required.

TransIT® Reagent for siRNA and miRNA

TransIT-TKO® Reagent and TransIT-siQUEST® Reagent are broad-spectrum siRNA transfection reagents that enable high efficiency siRNA delivery and knockdown of target gene expression in many cell types, including primary cells. TransIT-TKO® was introduced in 2001 and was the first commercially available siRNA transfection reagent. Cotransfection of siRNA and DNA is also feasible with TransIT-TKO® and has been tested across a variety of cell types. TransIT-siQUEST® was later developed to further supplement the number of cell lines that can be transfected with siRNA or miRNA. Transfections with either TransIT-TKO® or TransIT-siQUEST® does not require medium changes and can be carried out in serum-containing medium. Each unique formulation provides high-efficiency, broad-spectrum siRNA delivery.

TransIT® Reagent for Oligos

The TransIT®-Oligo Transfection Reagent provides maximal transfection performance for DNA and RNA oligonucleotides while maintaining low cellular toxicity. Oligonucleotides tested include:

  • phosphodiester DNA
  • phosphothioate DNA (ssDNA)
  • phosphothioate RNA (sRNA)
  • 2'-OMe RNA and 2'-OMe RNA/sDNA chimerics
  • Morpholino/DNA duplexes

TransIT®-Oligo provides all the advantages of the trusted TransIT® series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use and reproducibility.

Protocols

TransIT® Products

All TransIT® Transfection Reagents and Kits provide high performance and low toxicity. Moreover, they are all serum-compatible, which eliminates the need for a medium change. This simplicity enables a more streamlined, user-friendly protocol that leads to faster results.

A complete protocol is available for each TransIT® product. Each protocol is divided into the following sections:

  • Introduction: brief summary of specific product
  • Specifications: storage and warranty details
  • Materials:
    • Contents provided within the product package
    • Materials required but not supplied
  • Before You Start: suggestions to consider to ensure successful transfections
    • Seeding densities and cell confluency at transfection
    • Volumes of reagent and boost reagent (if applicable)
    • Nucleic acid considerations
    • Complex formation conditions
    • Cell culture conditions
    • Presence of antibiotics
    • Post-transfection incubation times
  • Transfection Protocol: specific transfection protocol details
    • Experimental set-up, including cell culture considerations
    • Table of recommended starting conditions for multiple plate formats
  • Troubleshooting Guide: recommendations to help troubleshoot problems
  • Related Products: list of related products
  • Reagent Agent®: information regarding the online transfection database
  • Contact Information

This section of the Mirus Transfectopedia® provides an overview of the TransIT® products, including schematic diagrams of workflows and a product decision tree. Please refer to the individual product protocols for more detailed experimental conditions.

The following TransIT® products and associated protocols are available for chemical transfection:

DNA and siRNA/miRNA

DNA

Broad Spectrum

Protein Production

Cell Line-Specific

RNA

Oligo

TransIT®-LT1 Video Protocol
General TransIT Schematic
TransIT-PRO Schematic

Electroporation

Mirus Bio has developed the Ingenio® Electroporation Solution and Kits to facilitate efficient and reliable delivery of nucleic acids to eukaryotic cells that are traditionally resistant to chemical transfection. Ingenio® is a broad-spectrum solution that supports high-efficiency electroporation with minimal toxicity. It replaces standard electroporation solutions, including phosphate-buffered saline (PBS) and serum-free media. Ingenio® is compatible with most conventional instruments and facilitates a wide range of applications that require nucleic acid delivery to cells. The Ingenio® solution is available both as a single product and as part of a complete kit with cuvettes and cell droppers.

Protocols for Electroporation

Ingenio® Kits and Solution are compatible with multiple conventional electroporation instruments including the Amaxa® Nucleofector®, Bio-Rad® Gene Pulser, and Harvard-BTX® electroporators. Ingenio® Kits and Solution can be used for electroporation with both exponential decay and square wave pulse conditions. Product protocols address pulse conditions in addition to cell culture and nucleic acid considerations.

Each Ingenio® protocol is divided into the following sections:

  • Introduction: brief summary of the specific product
  • Specifications: storage and warranty details
  • Materials:
    • Contents provided within the product package
    • Materials required but not supplied
  • Before You Start: suggestions to consider to ensure successful transfections
    • Cell density and passage number
    • Nucleic acid considerations for DNA and RNA
    • Optimization of pulse conditions
    • Exponential-decay wave form
    • Square wave form
    • Lonza-amaxa Nucleofector®
    • Post-electroporation incubation times
  • Electroporation Protocol: specific transfection protocol details
    • Experimental set-up, including cell seeding considerations
    • Preparation of Ingenio® Solution/nucleic acid/cell mixture
    • Electroporation details
    • Tables with recommended settings for particular cell types and instruments
  • Troubleshooting Guide: recommendations to help troubleshoot problems
  • Related Products: list of related products
  • Reagent Agent®: information regarding the online transfection database
  • Contact Information
Schematic Diagram for Ingenio Electroporation Workflow