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One focus. Transfection.

With nearly two decades of nucleic acid delivery experience, The Transfection Experts at Mirus Bio bring you a comprehensive Transfectopedia® that addresses various aspects of transfection. This manual includes:

  • A historical background of current and emerging transfection methodologies and applications
  • Tips to ensure successful transfection experiments from our bench to yours
  • Mirus Bio solutions for nucleic acid delivery

...from calcium phosphate precipitation to recent methods that are not only easier to perform but also more efficient and consistent.

The science behind transfection spans the humble beginning of gene delivery via calcium phosphate precipitation to recent methods that are not only easier to perform but also more efficient and consistent. Mirus Bio has been an integral player in transfection since the development of the first low-toxicity transfection formulation—TransIT®-LT1 in 1995. Over the years, Mirus has expanded the TransIT® line into a comprehensive platform of broad-spectrum and cell line–specific TransIT® Transfection Reagents and Kits. Mirus also added the Ingenio® Electroporation Kit and Solution for delivery of nucleic acids into a wide variety of cell types, some of them refractory to chemical transfection. A timeline of milestones and accomplishments since the inception of transfection is presented here; within this context, contributions to this field by The Transfection Experts are also highlighted. The Transfection Experts at Mirus continually promote innovation by developing new technologies to address multiple applications including virus production, stem cell research, large-scale protein production, and genome editing.

The Mirus Transfectopedia® is a compilation of transfection methodologies. It was designed for use as a guide to learn more about transfection and to help scientists think about, plan, and conduct experiments effectively. The objectives of the Mirus Transfectopedia® are to:

  • Present an overview of nucleic acid delivery methods, with emphasis on chemical transfection and electroporation.
  • Describe novel methodologies that may complement or even replace current transfection technologies.
  • Equip researchers with information on how to determine the best approach for delivering a given nucleic acid to various cell types.
  • Provide a collection of guidelines for procedure optimization
    (Tips From The Bench).
  • Highlight the comprehensive Mirus Bio platform of broad-spectrum and cell line–specific TransIT® Transfection Reagents and Kits and the Ingenio® Electroporation Kit and Solution.

The Transfection Experts at Mirus designed Transfectopedia® as a useful guide for all researchers. Our expert Technical Services Scientists always welcome your questions and feedback at: techsupport@mirusbio.com

What is Transfection?

Transfection is the delivery of nucleic acids into living cells through nonviral methods. The ultimate goal of transfection is to deliver nucleic acids to cells in order to study gene expression.

Learn more about transfection

One Focus Transfection
For Scientists. By Scientists.