To 1000L and Beyond
Removing AAV Scale-Up Bottlenecks
How Forge Biologics Simplified 1000L AAV Manufacturing with Transfection Complex Stabilization
30 min presentation
Key Takeaways for Process Developers Looking to Scale
- Reduce transfection bottlenecks at large scale
- Gain flexibility with longer, more forgiving complexation windows
- Boost productivity without changing your plasmids or ratios
- Minimize risk in scale-up from shake flask to GMP suites
The increase in maximum complexation time from an upper limit of 30 minutes out to two hours allows for improvement in process flexibility and robustness as we now have a significantly larger liquid addition time window.
See how Mason’s team at Forge Biologics:
4X
Expanded their complex formation window
2%
Cut their complexation volume by >50%
6X
Increased titers at 2 hours