Use Ingenio® Electroporation Solution with the Lonza-Amaxa® Nucleofector® II System for delivery of all nucleic acids; no further optimization is required
The Ingenio® Electroporation Kits and Solution can be used with any conventional electroporation instrument, including the Amaxa® Nucleofector® II System. With the Amaxa® Nucleofector®, use their recommended program or a preferred user-established protocol. The Ingenio® Solution will yield results similar to those obtained using the suggested Amaxa® solution and recommended program. Table 1 gives some examples.
Table 1. Results using Ingenio® Electroporation Solution on Amaxa® Nucleofector® II System, Compared to the Amaxa® Solution
| Cell Line |
Program |
Ingenio® Solution in Amaxa® Nucleofector® II |
Amaxa® solution in Amaxa® Nucleofector® II |
| Efficiency |
Solution |
Efficiency |
| Primary Human Keratinocytes |
T-018 |
30% |
Keratinocyte |
- |
| Primary MEFs |
A-023,
T-020 |
40% |
MEF I & II |
- |
| Primary Rat Cortical Neurons |
O-003 |
40% |
Neuron |
- |
| BHK-21 |
A-031 |
78% |
L |
- |
| CHO-K1 |
U-023 |
87% |
T |
71% |
| COS-7 |
W-001 |
60% |
R |
- |
| HEK-293 |
Q-001 |
79% |
V |
- |
| HeLa |
I-013 |
73% |
R |
- |
| HL-60 |
T-019 |
27% |
V |
32% |
| HUV-EC |
V-001 |
43% |
V |
- |
| Jurkat E6-1 |
X-001 |
50% |
V |
58% |
| K562 |
T-016 |
71% |
V |
64% |
| MCF-7 |
P-020 |
41% |
V |
42% |
| NIH-3T3 |
U-030 |
51% |
R |
- |
| Near-Diploid Immortalized Keratinocytes |
T-018 |
30% |
Keratinocyte |
- |
| PC-12 |
U-029 |
50% |
V |
- |
| SK-BR-3 |
E-009 |
35% |
C |
- |
| SK-N-MC |
S-020 |
52% |
V |
48% |
| THP-1 |
V-001 |
52% |
V |
51% |
| Vero |
V-001 |
65% |
V |
- |