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Tips from the Bench: Electroporation Tip

Use Ingenio® with Amaxa® Nucleofector® Device

Use Ingenio® Electroporation Solution with the Lonza-Amaxa® Nucleofector® II System for delivery of all nucleic acids; no further optimization is required

The Ingenio® Electroporation Kits and Solution can be used with any conventional electroporation instrument, including the Amaxa® Nucleofector® II System. With the Amaxa® Nucleofector®, use their recommended program or a preferred user-established protocol. The Ingenio® Solution will yield results similar to those obtained using the suggested Amaxa® solution and recommended program. Table 1 gives some examples.

Table 1. Results using Ingenio® Electroporation Solution on Amaxa® Nucleofector® II System, Compared to the Amaxa® Solution

Cell Line Program Ingenio® Solution in Amaxa® Nucleofector® II Amaxa® solution in Amaxa® Nucleofector® II
Efficiency Solution Efficiency
Primary Human Keratinocytes T-018 30% Keratinocyte -
Primary MEFs A-023,
40% MEF I & II -
Primary Rat Cortical Neurons O-003 40% Neuron -
BHK-21 A-031 78% L -
CHO-K1 U-023 87% T 71%
COS-7 W-001 60% R -
HEK-293 Q-001 79% V -
HeLa I-013 73% R -
HL-60 T-019 27% V 32%
HUV-EC V-001 43% V -
Jurkat E6-1 X-001 50% V 58%
K562 T-016 71% V 64%
MCF-7 P-020 41% V 42%
NIH-3T3 U-030 51% R -
Near-Diploid Immortalized Keratinocytes T-018 30% Keratinocyte -
PC-12 U-029 50% V -
SK-BR-3 E-009 35% C -
SK-N-MC S-020 52% V 48%
THP-1 V-001 52% V 51%
Vero V-001 65% V -

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