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Tips from the Bench: Electroporation Tip

Optimize Electroporation Using Ingenio® Electroporation Solution

Use an Exponential or Square Wave Form with the Ingenio® Electroporation Solution

Exponential decay and square wave forms are the most commonly used pulse types for mammalian cell electroporation. Ingenio® Electroporation Solution can be used with both exponential decay as well as square wave forms. Certain cell types transfect better with exponential decay while others respond better to square wave pulses. Therefore, empirical testing is required for each cell type. Regardless of the type of pulse used, titration of pulse strength is absolutely critical in ensuring high efficiency electroporation without causing undue loss of cell viability.

For Exponential Decay Pulse:
Test a voltage range of 200-300 V and a capacitance range of 800-1000 µF. First, keep the voltage constant at 220 V while varying capacitance in 100 µF increments starting at 750 µF. Following this, vary voltage in 10V increments starting at 200V keeping capacitance constant at the value determined from the capacitance titration.

For Square Wave Pulse:
For converting exponential decay conditions to square wave, use half the pulse length and increase the voltage by ~10%, while keeping capacitance the same. For further optimization, test 10V increments of voltage around the theoretically calculated pulse voltage.

Poster: Ingenio® Pulse Condition Optimization Details

Do Not Apply Additional Resistance to Your Electroporation Pulse. In addition to choices such as voltage, capacitance, and wave form, some electroporators also offer a choice of resistance (ohms). Using Ingenio® Electroporation Solution, the ideal resistance setting is: None, zero ohms (Ω) or ∞. If your electroporator requires a resistance setting other than zero, use the lowest possible resistance.

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