Label Plant miRNAs for Expression Profiling
The Label IT® miRNA Labeling Kits can be used to label any miRNA sample, including methylated plant miRNAs, making it an excellent choice for all miRNA microarray expression profiling applications. Endogenous plant miRNAs are methylated at their 3' ends (1). Many enzymatic miRNA labeling kits utilize a poly(A) polymerase tailing reaction to add nucleotides/labels to the 3' end of miRNAs, but poly(A) polymerase does not efficiently extend 3' methylated plant miRNAs (2) (Figure 1). In comparison, the Label IT® Reagents efficiently label plant miRNAs (or any nucleic acid) because they utilize a chemical alkylation reaction to internally label miRNAs that do not interfere with downstream hybridization applications (Figure 2).