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Tips from the Bench: In Vivo Tip

Deliver siRNA In Vivo

Use Hydrodynamic Tail Vein Injections to Deliver siRNA to the Mouse Liver

siRNA is effectively delivered to the mouse liver using hydrodynamic tail vein injection and TransIT®-QR Delivery Solution. Given the similar size and structure of siRNAs and duplex miRNAs, this method should also deliver duplex miRNAs to the mouse liver with similar efficiency.

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TransIT-QR Delivery Solution Effectively Delivers the Label IT Cy3 RNAi Delivery Control to Hepatocytes

TransIT®-QR Delivery Solution Effectively Delivers the Label IT® Cy®3 RNAi Delivery Control to Hepatocytes. TransIT®-QR Delivery Solution was used to deliver 25 µg of Label IT® Cy®3 RNAi Delivery Control (red) to a mouse using hydrodynamic delivery via the tail vein. Forty-five minutes post-injection the liver was harvested and sections were fixed then counterstained to stain the nuclei (blue) and to stain the actin (green).

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Endogenous Target Knockdowns Are Achievable in the Liver Using Hydrodynamic Delivery of siRNA.

Endogenous Target Knockdowns Are Achievable in the Liver Using Hydrodynamic Delivery of siRNA. Two different siRNAs directed against PPAR-alpha or a non-targeting control siRNA (40 µg each siRNA per mouse) were delivered via the hydrodynamic tail vein injection procedure to mice using the TransIT®-QR Hydrodynamic Delivery Solution. Twenty-four hours post-injection, the livers were harvested and the PPAR-alpha levels were determined using qRT-PCR and averaged for each group.

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