Transfect miRNAs In Vitro Using TransIT-X2® Dynamic Delivery System
TransIT-X2® Dynamic Delivery System is the reagent of choice for delivering miRNA, miRNA mimics or pre-miRNAs (see Figure 1). For miRNA transfection, follow the user protocol and simply substitute duplex miRNA for siRNA. TransIT-X2® delivers both plasmid DNA and/or miRNA efficiently and can be used for co-transfecting plasmid DNA and miRNA.
TransIT-TKO® and TransIT-siQUEST® Transfection Reagents can also effectively transfect duplex miRNAs into mammalian cells in culture. Ideally, all three reagents should be tested in parallel to determine the best solution for your application. Depending on the cell type, one reagent may have superior performance over the others. For cell-type specific recommendations, please consult the Reagent Agent® transfection database.