Compare Transfection Reagents Side-by-Side Using the Same Batch of Cells
Transfection results can vary day-to-day. In order to evaluate transfection reagents under optimal conditions, test each reagent side-by-side on the same day with the same batch of cells. Using historical comparisons (transfections performed on different days) to compare transfection reagents can lead to incorrect conclusions.
As an example, see the data presented in Figure 1. If a researcher compared transfection results generated using Reagent A on Day 1 to those obtained using Reagent B on Day 2 (Figure 1A), they would conclude that Reagent A was the better transfection reagent for these cells, which is incorrect.
When tested side-by-side, Reagent B outperformed Reagent A on both days (Figure 1B). Most importantly, the performance patterns were consistent from day-to-day although the overall luciferase activity varied.