TransIT® Lentivirus System

Optimized Transfection Reagent & Packaging Mix

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TransIT® Lentivirus System
(System includes TransIT®-Lenti Transfection Reagent, TransduceIT™ Transduction Reagent, and the Lentivirus Packaging Mix Powered by MISSION® Genomics)
Lentivirus Packaging Mix Powered by MISSION® Genomics
(Includes: Plasmid packaging mix only; Does not include transfection reagent.)
TransduceIT™ Transduction Reagent

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MIR 66505 RXNMIR 665534 RXN

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The TransIT® Lentivirus System combines the novel technologies of the TransIT®-Lenti Transfection Reagent with the Lentivirus Packaging Mix Powered by MISSION® Genomics. Titers of 10can be achieved with an optimized protocol. Whether you're a novice or an expert, you can rely on these benefits:

  • Produce 2-3x higher functional titers than Lipofectamine® 2000 & 3000
  • Achieve even higher titers and eliminate the need to concentrate
  • No media change required, single harvest
TransIT® Lentivirus System ConfigurationsClick to expand

Figures and Data

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Up to 100-fold Increase in Functional Lentivirus Titers

Up to 100-fold Increase in Functional Lentivirus Titers. Adherent 293T/17 cells were transfected in a 6-well plate with the pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix Powered by MISSION® Genomics or pLenti6.2-GW/EmGFP transfer vector and the ViraPower™ Lentiviral Packaging Mix (1:1 ration, 2 µg/well) with the following reagents: TransIT®-Lenti (3:1, vol:wt), Lipofectamine® 2000 (3:1), or Lipofectamine® 3000 (3:1:1). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

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Transfer Vector Selection Greatly Affects Functional Lentivirus Titers

Transfer Vector Selection Greatly Affects Functional Lentivirus Titers. Adherent 293T/17 cells were transfected in a 6-well plate with one of three transfer vectors: MISSION® pLKO.1-puro-CMV-TurboGFP™, ViraPower™ pLenti6.2-GW/EmGFP, or Precision Lenti-ORF™ RFP and the Lentivirus Packaging Mix powered by MISSION® Genomics) (1:1 ratio, 2 µg/well) using the TransIT®-Lenti Transfection Reagent (3:1, vol:wt). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

Resources

Specifications

Storage Conditions:
Lentivirus Packaging Mix Powered by MISSION® Genomics: Store at -20°C
Lentivirus Packaging Mix Powered by MISSION® Genomics: Multiple storage conditions - see individual bottles for specific recommendations
TransIT® Lentivirus System: Store at -20°C
TransduceIT™ Transduction Reagent: Store at -20°C

Product Guarantee:
Lentivirus Packaging Mix Powered by MISSION® Genomics: 1 year
TransIT® Lentivirus System:
TransduceIT™ Transduction Reagent: 6 months

Usage Statement:
All Configurations: For Research Use Only.

Animal Origin Statement:
All Configurations: This product is animal origin free.

Technical Product Literature


Additional Resources

COA Lookup

Download Certificates of Analysis

COO Lookup

Download Certificate of Origin

Testimonials

Excellent technical support, they are very knowledgeable.
He Song Sun
University of Toronto Scarborough - Cell and Molecular Biology