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NEW! TransIT-VirusGEN® SELECT Transfection Reagent

Large Scale Virus Production for Preclinical and Early Phase Clinical Trials

To inquire about bulk pricing, please call 888-530-0801
International inquiries please call +1-608-441-2852

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MIR 673030 ml

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All TransIT-VirusGEN® reagents are designed to enhance delivery of packaging and transfer vector DNA to suspension and adherent HEK 293 cell types in order to increase production of recombinant lentivirus and adeno-associated virus (AAV).

 

TransIT-VirusGEN® SELECT Transfection Reagent is identical in formulation to our research-grade TransIT-VirusGEN® and includes extensive quality release testing that provides the assurance needed when using ancillary materials to manufacture viral vectors for preclinical and early phase clinical trials.

 

TransIT-VirusGEN® SELECT outperforms PEI and PEIpro® reagents and offers a simplified, cost-effective workflow, making it the optimal choice for large-scale virus production.

 

  • High Performance  Efficient DNA delivery for large-scale production of high-titer viral vectors 
  • Extensively Characterized – Tested to confirm formulation identity, sterility, low endotoxin levels, and absence of mycoplasma 
  • Reliable – Exceptional lot-to-lot consistency   
  • Flexible – Compatible with different recombinant virus platforms and cell culture systems
  • Animal Origin Free – Fully synthetic transfection reagent formulation
    [Example Certificate of Origin | Example Certificate of Analysis]
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TransIT-VirusGEN® Product Family

TransIT-VirusGEN Biotherapeutic Pipeline

Sample TransIT-VirusGEN® SELECT Transfection Reagent for gene and cell therapy manufacturing

Sample TransIT-VirusGEN® Transfection Reagent for research and development

Figures and Data

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TransIT-VirusGEN SELECT Suspension and Adherent PEI Comparison

TransIT-VirusGEN® SELECT Outperforms Competitor 25 kDa linear PEI and PEIpro® Transfection Reagents in Suspension and Adherent Cell Culture Systems. 
Lentivirus was produced using (A) suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium or (C) adherent 293T/17 cells grown in DMEM + 10% FBS and transfected with 3rd generation vectors pLKO.1-puro-CMV-TurboGFP™ transfer vector (Sigma) and ViraSafe™ Pantropic Packaging mix (pRSV-Rev, pCMV-VSV-G, pCgpV, Cell Bio Labs) at a 3:0.5:0.5:2 DNA ratio, 1 μg/ml total plasmid DNA, using the TransIT-VirusGEN® SELECT Transfection Reagent (3:1, vol:wt), 25 kDa linear PEI (4:1, Polysciences) or PEIpro® (2:1, Polyplus Transfection). Virus-containing supernatant was used to transduce 293T/17 cells and GFP expression was measured at 72 hours post-transduction using a Guava® easyCyte™ 5HT Flow Cytometer.

AAV2 was produced using (B) suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium or (D) adherent 293T/17 cells grown in DMEM + 10% FBS and transfected using pAAV-hrGFP, pAAV-RC, and pAAV-Helper plasmids (1:1:1 DNA ratio, 1.5 μg/ml plasmid DNA, Agilent Technologies) using TransIT-VirusGEN® SELECT Transfection Reagent (2:1, vol:wt), 25 kDa linear PEI (2:1, Polysciences) or PEIpro® (2:1 or 4:1, Polyplus Transfection). Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction using a Guava® easyCyte™ 5HT Flow Cytometer. For both lentivirus and AAV, functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the range of triplicate wells.

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TransIT-VirusGEN SELECT Lot-to-Lot Variability
Lot-to-Lot Consistency with the TransIT-VirusGEN® SELECT Transfection Reagent.
(A) Lentivirus was produced using suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium and transfected with 3rd generation vectors pLKO.1-puro-CMV-TurboGFP™ transfer vector (Sigma) and ViraSafe™ Pantropic Packaging mix (pRSV-Rev, pCMV-VSV-G, pCgpV, Cell Bio Labs) at a 3:0.5:0.5:2 DNA ratio, 1 ug/ml total plasmid, using the TransIT-VirusGEN® SELECT Transfection Reagent (3:1, vol:wt). Virus containing supernatant was used to transduce 293T/17 cells and GFP expression was measured at 72 hours post-transduction using a Guava® easyCyte™ 5HT Flow Cytometer.

(B) AAV2 was produced using suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium and transfected using pAAV-hrGFP, pAAV-RC, and pAAV-Helper plasmids (1:1:1 DNA ratio, 1.5 µg/ml, Agilent Technologies) using TransIT-VirusGEN® SELECT Transfection Reagent (2:1, vol:wt). Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction using a Guava® easyCyte™ 5HT Flow Cytometer. For both lentivirus and AAV, functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.
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TransIT-VirusGEN SELECT Multiple Filtrations

TransIT-VirusGEN® SELECT Transfection Reagent can be Filtered Multiple Times with No Effect on Reagent Performance. 
(A) Lentivirus was produced using suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium and transfected with 3rd generation vectors pLKO.1-puro-CMV-TurboGFP™ transfer vector (Sigma) and ViraSafe™ Pantropic Packaging mix (pRSV-Rev, pCMV-VSV-G, pCgpV, Cell Bio Labs) at a 3:0.5:0.5:2 DNA ratio, 1 ug/ml total plasmid, using the TransIT-VirusGEN® SELECT Transfection Reagent (3:1, vol:wt) that was filtered through a 0.22 um polyethersulfone (PES) filter unit (Millipore Sigma) for the indicated number of times. Virus-containing supernatant was used to transduce 293T/17 cells and GFP expression was measured at 72 hours post-transduction using a Guava® easyCyte™ 5HT Flow Cytometer.

(B) AAV2 was produced using suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium and transfected using pAAV-hrGFP, pAAV-RC, and pAAV-Helper plasmids (1:1:1 DNA ratio, 1.5 µg/ml, Agilent Technologies) using TransIT-VirusGEN® SELECT Transfection Reagent (2:1, vol:wt). Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction using a Guava® easyCyte™ 5HT Flow Cytometer. For both lentivirus and AAV functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.

 

Resources

For a Certificate of Origin (COO) and/or Certificate of Analysis (COA) for this product, please contact techsupport@mirusbio.com.

Specifications

Storage Conditions:
All Configurations: Store at -10 to -30°C

Product Guarantee:
All Configurations: 6 months

Technical Product Literature


Additional Resources

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