TransIT®-VirusGEN™ Transfection Reagent

Ideal for Recombinant Adeno-associated Virus Production

  Product No. Quantity Price Add to Cart
TransIT®-VirusGEN™ Transfection Reagent
  MIR 6703 0.3 ml $126.00
  MIR 6704 0.75 ml $315.00
  MIR 6700 1.5 ml $499.00
  MIR 6705 5 x 1.5 ml $2,169.00
  MIR 6706 10 x 1.5 ml $3,990.00

Product Overview

TransIT®-VirusGEN™ Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent and suspension HEK 293T cell types and increase recombinant adeno-associated virus (AAV) and lentivirus production.

  • Reliable – Consistent high virus titer production 
  • Scalable – Efficient across different formats
  • Flexible – Address different virus and cell culture systems
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Figures and Data

TransIT-VirusGEN Outperforms Competitor Reagents in Suspension & Adherent AAV Cell Cultures
TransIT®-VirusGEN™ Outperforms Competitor Reagents in Suspension & Adherent AAV Cell Cultures. Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio, 1.5 µg/ml, Agilent Technologies) with the following reagents: TransIT®-VirusGEN™ (2:1, vol:wt), Lipofectamine® 2000 (3:1, Thermo Fisher), Lipofectamine® 3000 (3:3:1, Thermo Fisher) or 25 kDa PEI (4:1, Polysciences). Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.
TransIT-VirusGEN Outperforms Competitor Reagents in Suspension and Adherent Lentivirus Cell Cultures
TransIT®-VirusGEN™ Outperforms Competitor Reagents in Suspension and Adherent Lentivirus Cell Cultures. Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION® (1:1 ratio, 1 µg/ml)  with the following reagents: TransIT®-VirusGEN™ (3:1, vol:wt), Lipofectamine® 2000 (2:1 (A) or 3:1 (B), Thermo Fisher), Lipofectamine® 3000 (2:2:1 (A) or 3:3:1 (B), Thermo Fisher) or 25 kDa PEI (4:1, Polysciences). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.
TransIT-VirusGEN Enables Broad Scalability (AAV)
TransIT®-VirusGEN™ Enables Broad Scalability (AAV). Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio, 1.5 total µg/ml, Agilent Technologies) using the TransIT®-VirusGEN™ Transfection Reagent (2:1 reagent-to-DNA ratio (vol:wt), Mirus) at the indicated volumes perculture vessel. Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction by flow cytometry. Functional titers were measured from virus dilutions with less than 20% GFP positive cells.  Error bars represent the standard deviation of triplicate wells, flasks, or dishes. 

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Specifications

Storage Conditions:
All Configurations: Store at -20°C

Product Guarantee:
All Configurations: 6 months

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