Experience the transfection breakthrough. Achieve superior transfections with an innovative polymeric system that efficiently delivers both DNA and RNA out of the endosome and into the cytoplasm overcoming a critical barrier to nucleic acid delivery. The
–Cutting Edge Delivery of Plasmid DNA and small RNAs (siRNA, miRNA and CRISPR guide RNA)
IT-X2® Dynamic Delivery System Enables Superior Gene Expression in a Variety of Cell Types.
IT-X2® Dynamic Delivery System and Lipofectamine® 2000 Transfection Reagent were used to transfect plasmid DNA encoding luciferase into 41 different cell types at three reagent-to-DNA ratios. Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. Head-to-head comparisons at optimized ratios illustrate superior or equal luciferase expression using
IT-X2® in 36 of 41 cell types; 17 cell types that had expression levels 2-fold higher are denoted with ‡.
Visualization of High GFP Expression using Trans
IT-X2® Dynamic Delivery SystemFigure 2:
High GFP Transfection Efficiency in Multiple Cell Lines and Primary Cells Using Trans
IT-X2® SystemFigure 3:
Functional Co-delivery of Plasmid DNA and siRNA using Trans
IT-X2® SystemFigure 4: Trans
IT-X2® Dynamic Delivery System Achieves Higher Knockdown than Lipofectamine® 2000Figure 5:
Effective miRNA Delivery using Trans
IT-X2® Yields Decreased Levels of PTK9 mRNAFigure 6:
High efficiency CRISPR genome editing with Trans