TransIT-X2® Dynamic Delivery System for
Broad Spectrum Transfection

An advanced system for delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components

  Product No. Quantity Price Add to Cart
TransIT-X2® Dynamic Delivery System
  MIR 6003 0.3 ml $129.00
  MIR 6004 0.75 ml $322.00
  MIR 6000 1.5 ml $536.00
  MIR 6005 5 x 1.5 ml $2,327.00
  MIR 6006 10 x 1.5 ml $4,279.00

Product Overview

Experience the transfection breakthrough. Achieve superior transfections with an innovative polymeric system that efficiently delivers both DNA and RNA out of the endosome and into the cytoplasm overcoming a critical barrier to nucleic acid delivery. The TransIT-X2® Dynamic Delivery System gives researchers:

icon Efficiency – Exceptional Broad Spectrum Transfection
icon Versatility – Cutting Edge Delivery of Plasmid DNA and small RNAs (siRNA, miRNA and CRISPR guide RNA)
icon Technology – Novel, Non-Liposomal, Polymeric Delivery

Learn more about using TransIT-X2® for CRISPR/Cas9 genome editing

 

TransIT-X2® System Enables Superior Gene Expression in a Variety of Cell Types

‡ Cell types with >2-fold greater expression in head-to-head comparisons
Click here for cell-type specific transfection protocol recommendations
 

TransIT-X2® Dynamic Delivery System Enables Superior Transfection in a Variety of Cell Types. TransIT-X2® Dynamic Delivery System and Lipofectamine® 2000 Transfection Reagent were used to transfect plasmid DNA encoding luciferase into 41 different cell types at three reagent-to-DNA ratios. Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. Head-to-head comparisons at optimized ratios illustrate superior or equal luciferase expression using TransIT-X2® in 36 of 41 cell types; 17 cell types that had expression levels 2-fold higher are denoted with ‡.

Figures and Data

TransIT-X2 Dynamic Delivery System Outperforms Lipofectamine Reagents

TransIT-X2® Dynamic Delivery System Outperforms Lipofectamine® Reagents. A549 (A) or MDCK (B) cells were transfected with luciferase encoding plasmid DNA using either TransIT-X2 (Mirus Bio), Lipofectamine® 2000 (Thermo Fisher Scientific) or Lipofectamine® 3000 (Thermo Fisher Scientific) for 24 hours at indicated reagent-to-DNA ratios or reagent-to-P3000™-to-DNA ratio. Transfection was measured by luciferase activity using a conventional assay. Cytotoxicity was assessed by quantifying the LDH released from the cytosol of damaged cells compared to cells alone. Higher transgene expression and lower cytotoxicity was observed in cells transfected with TransIT-X2 at optimal ratios compared to cells transfected with Lipofectamine® 2000 or Lipofectamine® 3000.

Visualization of High GFP Expression Using TransIT-X2 Dynamic Delivery System

Visualization of High GFP Expression Using TransIT-X2® Dynamic Delivery System. TransIT-X2® Dynamic Delivery System was used to transfect plasmid DNA encoding EGFP into A549, CHO-K1, HepG2, LNCaP, MDCK, PC12, primary human mammary epithelial cells (HMEC) and normal human dermal fibroblasts (NHDF). Transfections were performed in 35 mm MatTek dishes using 4-8 µl of TransIT-X2® to deliver 2 µg of DNA. Images (32X) were captured at 48 hours post-transfection using a Zeiss Axiovert S100 inverted fluorescence microscope.

* indicates primary cell types

High GFP Transfection Efficiency in Multiple Cell Lines and Primary Cells Using TransIT-X2 System

High GFP Transfection Efficiency in Multiple Cell Lines and Primary Cells Using TransIT-X2® Dynamic Delivery System. TransIT-X2® Dynamic Delivery System was used to transfect plasmid DNA encoding EGFP into A549, CHO-K1, Hep G2, MDCK, LNCaP, PC-12, primary human mammary epithelial cells (HMEC) and normal human dermal fibroblasts (NHDF). Transfections were performed in 96-well plates using 0.2-0.4 µl of TransIT-X2® to deliver 0.1 µg of DNA (2:1, 3:1 or 4:1 reagent: DNA ratio). Triplicate wells were assayed 48 hours post-transfection using guava easyCyte™ 5HT Flow Cytometer.

*indicates primary cell types

Product Resources

Specifications

Storage Conditions:
All Configurations: Store at -20°C

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


Additional Resources

COA Lookup

Download Certificates of Analysis

COO Lookup

Download Certificate of Origin

Feedback

See what others are saying about the TransIT-X2® Dynamic Delivery System...

"I was recently tasked with developing a CRISPR protocol for primary and bone-derived cell lines. TransIT-X2® was simple to use, 2-3 times better for transfection and much gentler on my cells than other products! I feel I have hit the jackpot and have already passed this exciting information on to my colleagues."
- Dr. Joshua Chou, Harvard School of Dental Medicine

"The TransIT-X2® Dynamic Delivery System outperformed all other transfection reagents we have tested for DNA transfection of our C2C12 mouse myoblast cell line. In addition, TransIT-X2® was also less toxic."
- Dr. Guangwei Du, Texas Medical Center

"We work on non-small cell lung cancer (NSCLC) which is an adherent cell culture line. Previously, we have tested many transfection products from several companies without much success, but the TransIT-X2® Dynamic Delivery System works very well with NSCLC using my protocol."
- Dr. Luo Wang, University of Michigan Comprehensive Cancer Center

"We recently tested the TransIT-X2® Dynamic Delivery System head-to-head against Lipofectamine® 2000 for DNA transfection of NIH-3T3 fibroblasts and the breast cancer cell line ZR-75-1. We observed higher efficiency and less toxicity when using TranslT-X2. We are also pleased to hear that TranslT-X2 will be offered in similar volume configurations to Lipofectamine® 2000."
- Dr. Edwin Li, Assistant Professor, Saint Joseph's University

"We were pleased with the performance and low toxicity of the TransIT-X2® Dynamic Delivery System when transfecting our renal carcinoma cell line 786-0."
- Sathish Padi, North Dakota State University

A549 TransIT-X2

"The TransIT-X2® Dynamic Delivery System performed better than our regular transfection reagent (Polyjet™) for delivering DNA into the hard to transfect A549 cell line. TransIT-X2® was able to show protein expression compared to Polyjet™ which failed to produce detectable levels of protein containing V5 tag."
- Dr. Jason Liggett and Kyung-Won Min, Baek Lab, University of Tennessee

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