TransIT-X2® Dynamic Delivery System

An advanced system for delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components

  Product No. Quantity Price Add to Cart
TransIT-X2® Dynamic Delivery System
  MIR 6003 0.3 ml $126.00
  MIR 6004 0.75 ml $315.00
  MIR 6000 1.5 ml $525.00
  MIR 6005 5 x 1.5 ml $2,281.00
  MIR 6006 10 x 1.5 ml $4,195.00

Product Overview

Experience the transfection breakthrough. Achieve superior transfections with an innovative polymeric system that efficiently delivers both DNA and RNA out of the endosome and into the cytoplasm overcoming a critical barrier to nucleic acid delivery. The TransIT-X2® Dynamic Delivery System gives researchers:

icon Efficiency – Exceptional Broad Spectrum Transfection
icon Versatility – Cutting Edge Delivery of Plasmid DNA and small RNAs (siRNA, miRNA and CRISPR guide RNA)
icon Technology – Novel, Non-Liposomal, Polymeric Delivery

Learn more about using TransIT-X2® for CRISPR/Cas9 genome editing

 

TransIT-X2® System Enables Superior Gene Expression in a Variety of Cell Types

‡ Cell types with >2-fold greater expression in head-to-head comparisons
Click here for cell-type specific transfection protocol recommendations
 

TransIT-X2® Dynamic Delivery System Enables Superior Gene Expression in a Variety of Cell Types. TransIT-X2® Dynamic Delivery System and Lipofectamine® 2000 Transfection Reagent were used to transfect plasmid DNA encoding luciferase into 41 different cell types at three reagent-to-DNA ratios. Luciferase expression was compared at 24 hours post-transfection using a standard luciferase assay. Head-to-head comparisons at optimized ratios illustrate superior or equal luciferase expression using TransIT-X2® in 36 of 41 cell types; 17 cell types that had expression levels 2-fold higher are denoted with ‡.

Figures and Data

Visualization of High GFP Expression Using TransIT-X2 Dynamic Delivery System

Visualization of High GFP Expression Using TransIT-X2® Dynamic Delivery System. TransIT-X2® Dynamic Delivery System was used to transfect plasmid DNA encoding EGFP into A549, CHO-K1, HepG2, LNCaP, MDCK, PC12, primary human mammary epithelial cells (HMEC) and normal human dermal fibroblasts (NHDF). Transfections were performed in 35 mm MatTek dishes using 4-8 µl of TransIT-X2® to deliver 2 µg of DNA. Images (32X) were captured at 48 hours post-transfection using a Zeiss Axiovert S100 inverted fluorescence microscope.

* indicates primary cell types

High GFP Transfection Efficiency in Multiple Cell Lines and Primary Cells Using TransIT-X2 System

High GFP Transfection Efficiency in Multiple Cell Lines and Primary Cells Using TransIT-X2® Dynamic Delivery System. TransIT-X2® Dynamic Delivery System was used to transfect plasmid DNA encoding EGFP into A549, CHO-K1, Hep G2, MDCK, LNCaP, PC-12, primary human mammary epithelial cells (HMEC) and normal human dermal fibroblasts (NHDF). Transfections were performed in 96-well plates using 0.2-0.4 µl of TransIT-X2® to deliver 0.1 µg of DNA (2:1, 3:1 or 4:1 reagent: DNA ratio). Triplicate wells were assayed 48 hours post-transfection using guava easyCyte™ 5HT Flow Cytometer.

*indicates primary cell types

Functional Co-delivery of Plasmid DNA and siRNA Using TransIT-X2 System

Functional Co-delivery of Plasmid DNA and siRNA Using the TransIT-X2® Dynamic Delivery System. TransIT-X2® Dynamic Delivery System was used to transfect plasmid Cy®5 labeled DNA encoding nuclear YFP and Cy®3 labeled siRNA into HeLa cells. Transfection was performed in 6-well plates with Poly-L-Lysine (PLL) coated coverslips using 4 µl of TransIT-X2® to deliver 2 µg of DNA and 25 nM siRNA (2:1 reagent:DNA ratio). Actin cytoskeleton was stained using Alexa Fluor® 350 Phalloidin. Images (63X) were captured at 24 hours post-transfection using a Nikon A1R confocal microscope. Image key: yellow (nuclear YFP), blue (Cy®5 labeled DNA), red (Cy®3 labeled siRNA), green (actin cytoskeleton).

Product Resources

Specifications

Storage Conditions:
All Configurations: Store at -20°C

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


Additional Resources

COA Lookup

Download Certificates of Analysis

COO Lookup

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Feedback

See what others are saying about the NEW! TransIT-X2® Dynamic Delivery System...

"I was recently tasked with developing a CRISPR protocol for primary and bone-derived cell lines. TransIT-X2® was simple to use, 2-3 times better for transfection and much gentler on my cells than other products! I feel I have hit the jackpot and have already passed this exciting information on to my colleagues."

- Dr. Joshua Chou, Harvard School of Dental Medicine

"The TransIT-X2® Dynamic Delivery System outperformed all other transfection reagents we have tested for DNA transfection of our C2C12 mouse myoblast cell line. In addition, TransIT-X2® was also less toxic."
- Dr. Guangwei Du, Texas Medical Center

"We work on non-small cell lung cancer (NSCLC) which is an adherent cell culture line. Previously, we have tested many transfection products from several companies without much success, but the TransIT-X2® Dynamic Delivery System works very well with NSCLC using my protocol."
- Dr. Luo Wang, University of Michigan Comprehensive Cancer Center

"We recently tested the TransIT-X2® Dynamic Delivery System head-to-head against Lipofectamine® 2000 for DNA transfection of NIH-3T3 fibroblasts and the breast cancer cell line ZR-75-1. We observed higher efficiency and less toxicity when using TranslT-X2. We are also pleased to hear that TranslT-X2 will be offered in similar volume configurations to Lipofectamine® 2000."
- Dr. Edwin Li, Assistant Professor, Saint Joseph's University

"We were pleased with the performance and low toxicity of the TransIT-X2® Dynamic Delivery System when transfecting our renal carcinoma cell line 786-0."
- Sathish Padi, North Dakota State University

A549 TransIT-X2

"The TransIT-X2® Dynamic Delivery System performed better than our regular transfection reagent (Polyjet) for delivering DNA into the hard to transfect A549 cell line. TransIT-X2® was able to show protein expression compared to Polyjet which failed to produce detectable levels of protein containing V5 tag."
- Dr. Jason Liggett and Kyung-Won Min, Baek Lab, University of Tennessee

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