TransIT®-Insect Transfection Reagent

Superior transient transfection for high yield baculovirus titers in insect cells

  Product No. Quantity Price Add to Cart
TransIT®-Insect Transfection Reagent
  MIR 6104 0.4 ml $130.00
  MIR 6100 1 ml $270.00
  MIR 6105 5 x 1 ml $1,167.00
  MIR 6106 10 x 1 ml $2,145.00

Product Overview

Insect cell expression is a platform used to produce proteins with simple post-translational modifications. Transient transfection and recombinant baculovirus production are commonly used methods for insect cell expression.TransIT®-Insect Transfection Reagent is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types that offers:

  • Exceptional DNA Delivery – In insect cell types including Sf9, High Five™ and S2
  • High Baculovirus Production – Ideal for baculovirus expression in insect cells
  • Serum Compatibility – Non-liposomal, animal-origin free formulation that eliminates media change
  • Better Value – Low reagent amounts required per transfection

Mirus Bio also offers flashBAC™ Baculovirus Expression Systems and pOET Insect and BacMam Transfer Plasmids for baculovirus production and protein expression in insect or mammalian cells.

Figures and Data

Efficient Transfection of Baculovirus Genomic DNA Using TransIT-Insect Reagent

Efficient Transfection of Baculovirus Genomic DNA Using TransIT®-Insect Reagent. Transfections were performed in 6-well plates with 5 x 105 Sf9 cells per well using TransIT®-Insect Transfection Reagent  at the reagent-to-total DNA ratio of 3:1 (µl:µg). Cells were co-transfected with 0.5 µg of ProGreen™ baculovirus genomic vector DNA (AB Vector) encoding green-fluorescent protein (GFP) and 0.1 µg of pVL1393 transfer vector (AB Vector). (A) Fluorescence and phase contrast images were taken at 6 days post-transfection using a Zeiss S100 fluorescent microscope. Merge shown in (B).

TransIT-Insect Outperforms Competitor Transfection Reagents

TransIT®-Insect Outperforms Competitor Transfection Reagents. Insect cell lines (A) Sf9, (B) High Five™, and (C) Drosophila S2 cells were transfected in 96-well plates with 0.1 µg of a luciferase expression plasmid driven by an hr5 enhancer/IE1 promoter using the designated reagent at the indicated reagent-to-DNA ratios (µl: µg). Luciferase expression was measured at 48 hours post-transfection. Sf9 and High Five™ cells were cultured and transfected in serum-free media formulations; S2 cells were in serum containing medium.  Error bars represent the standard error of the mean for triplicate wells.

Superior Recombinant Protein Expression in High Five Cells Using TransIT-Insect

Superior Recombinant Protein Expression in High Five™ Cells Using TransIT®-Insect. High Five™ cells were transfected in 6-well plates with 2.5 µg of a GFP expression plasmid driven by an hr5 enhancer/IE1 promoter using the designated reagent at the indicated reagent-to-DNA ratios (µl: µg). Total soluble cell lysates were prepared from cells 72 hours post-transfection. Lysates from 100 µl culture were analyzed by SDS-PAGE and Coomassie blue staining; cells alone (untransfected) is shown as control. Expressed GFP containing 6X His, S, and HSV tags (~38 kDa) was clearly detected in the lysate from the cells that were transfected (*) with the highest level of expression observed at TransIT®-Insect:DNA ratio of 2:1.

Product Resources

Specifications

Storage Conditions:
All Configurations: Store at -20°C

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


Additional Resources

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Feedback

The buzz about TransIT®-Insect Reagent...

"Our lab successfully tested TransIT®-Insect Transfection Reagent for generating recombinant baculovirus in insect cells. Using TransIT®-Insect with multiple BEVS we were able to generate high-titer baculovirus that resulted in consistently higher protein expression in High Five™ and Sf9 cells compared to Cellfectin® II (Life Technologies)."
-Dr. Linda Lua (Director), The University of Queensland - Protein Expression Facility

"Our lab tested TransIT®-Insect for virus production in Sf9 cells. It performed better than Cellfectin® II that we currently use for transfecting these cells. Cytopathic effects (CPE) of the baculovirus were observed after 5 days with as little as 0.5 µg DNA per well of a 6-well plate. When using Cellfectin® and other reagents, we haven’t seen any results using the same amount of input DNA."
-Eric Carlin, Florida Gulf Coast University

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