TransIT®-BrCa Transfection Reagent

Superior DNA transfection of breast cancer cell types

  Product No. Quantity Price Add to Cart
TransIT®-BrCa Transfection Reagent
  MIR 5504 0.4 ml $230.00
  MIR 5500 1 ml $349.00
  MIR 5505 5 x 1 ml $1,521.00
  MIR 5506 10 x 1 ml $2,813.00

Product Overview

TransIT®-BrCa Transfection Reagent is the first dedicated breast cancer cell line specific reagent available to researchers. While it delivers efficiently to many breast cancer cell types, it also maintains low cellular toxicity resulting in reduced experimental biases.

  • Broad Spectrum DNA Delivery – Achieve high expression levels in multiple breast cancer cell types
  • Formulated for Low Cellular Toxicity – Maintain cell density and reduce experimental biases due to toxicity
  • Superior Transfection Efficiency – TransIT®-BrCa outperforms Lipofectamine® 2000, in various breast cancer cell lines
 

Figures and Data

TransIT-BrCa Transfection Reagent Exhibits Higher Luciferase Expression in Breast Cancer Cells Compared to Other Transfection Reagents

TransIT®-BrCa Transfection Reagent Exhibits Higher Luciferase Expression in Breast Cancer Cells Compared to Other Transfection Reagents. Breast Cancer cell lines, MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D, were transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression was measured at 24 hours post-transfection using a standard assay. Error bars represent the standard deviation of triplicate wells.

High Efficiency Delivery of Plasmid DNA in Breast Cancer Cell Types

High Efficiency Delivery of Plasmid DNA in Breast Cancer Cell Types. TransIT®-BrCa Transfection Reagent was used to transfect plasmid DNA encoding GFP into MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D breast cancer cell lines. Transfections were performed in 35 mm MatTek dishes using 4 µl of TransIT®-BrCa Transfection Reagent to deliver 2 µg of DNA (2:1 reagent:DNA ratio). Images (32X) were captured at 48 hours post-transfection using a Zeiss Axiovert S100 inverted fluorescence microscope.

TransIT-BrCa Transfection Reagent Yields High Efficiency Plasmid DNA Transfection

TransIT®-BrCa Transfection Reagent Yields High Efficiency Plasmid DNA Transfection. TransIT®-BrCa Transfection Reagent was used to transfect plasmid DNA encoding GFP into MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D breast cancer cell lines. Transfections were performed  in 24-well plates using 1-1.5 µl of TransIT®-BrCa Transfection Reagent to deliver 0.5 µg of DNA (2:1 and 3:1, reagent:DNA ratio). Triplicate wells were assayed 48 hours post-transfection on a Guava® HT easyCyte™ flow cytometer.

Product Resources

Specifications

Storage Conditions:
All Configurations: Store at -20°C

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


Additional Resources

COA Lookup

Download Certificates of Analysis

Feedback

This product does not yet have related testimonials.

Watch Us On YouTube
Close