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TransIT®-3T3 Transfection Kit

IMPORTANT NOTICE: The TransIT®-3T3 Transfection Kit was discontinued in Dec'2014. Our primary recommendation for 3T3 cell types is the new broad-spectrum TransIT-X2® Dynamic Delivery System, that offers the following benefits to researchers:

  • Higher gene expression than TransIT®-3T3 Kit (see data below)
  • Efficient delivery of siRNA/miRNA in addition to plasmid DNA
  • Easier protocol; no boost reagent required
  • Better broad-spectrum value

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TransIT-X2® Dynamic Delivery System, more information>>
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TransIT-X2 Exhibits Superior Gene Expression in NIH-3T3 Cells

TransIT-X2® Exhibits Superior Gene Expression in NIH-3T3 Cells. NIH-3T3 cells were transfected with a luciferase expression plasmid using the TransIT®-3T3 Transfection Kit or the TransIT-X2® Dynamic Delivery System at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression was measured at 24 hours post-transfection.  Error bars represent the standard deviation of triplicate wells.