TransIT®-Lenti Transfection Reagent

Ideal for Recombinant Lentivirus Production

  Product No. Quantity Price Add to Cart
TransIT®-Lenti Transfection Reagent
  MIR 6603 0.3 ml $120.00
  MIR 6604 0.75 ml $300.00
  MIR 6600 1.5 ml $475.00
  MIR 6605 5 x 1.5 ml $2,066.00
  MIR 6606 10 x 1.5 ml $3,800.00
TransduceIT™ Transduction Reagent
  MIR 6620 1 ml $25.00

Product Overview

TransIT®-Lenti Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent HEK 293T cell types and increase recombinant lentivirus production.

  • High Performance – Provide up to eight-fold higher functional titers
  • Simple Protocol – No media change required, single harvest
  • Animal Origin Free  Regulatory friendly
 
Glossary of Lentivirus Terms Click to expand
Overview of Recombinant Lentivirus ProductionClick to expand
 

Figures and Data

High Functional Titers with TransIT-Lenti Transfection Reagent

High Functional Titers with TransIT®-Lenti Transfection Reagent. Adherent 293T/17 cells were transfected in a 6-well plate with pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION® (1:1 ratio, 2 µg/well) with the following reagents: TransIT®-Lenti (3:1, vol:wt), Lipofectamine® 2000 (3:1), Lipofectamine® 3000 (3:1:1), 25 kDa PEI (6:1), or CaPO4 precipitation (4 µg pDNA/well). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

High Transduction Efficiency in Neuron Cells with Unconcentrated Lentivirus Using TransIT-Lenti

High Transduction Efficiency with Unconcentrated Lentivirus Using TransIT®-Lenti. (A) Lentivirus was produced with the TransIT®-Lenti Transfection Reagent (3:1, vol:wt) or Lipofectamine® 2000 using the MISSION® vectors (pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION®). The supernatant was harvested, filtered (0.45 µm), and frozen. Lentivirus transductions were performed 5 days post-plating with iCell® Motor Neurons (Cellular Dynamics International). For bothTransIT-Lenti and Lipofectamine® 2000, one microliter of unconcentrated supernatant was added per well of a 96-well plate. GFP efficiency was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent the SEM of duplicate wells. (B) iCell® Motor Neurons were plated in Ibidy 35mm dishes and transduced withlentivirus produced using the TransIT®-Lenti Transfection Reagent and MISSION® vectors. Images were captured at 72 hours post-transduction with a Zeiss Axiovert S100 inverted fluorescence microscope using a 63X objective under oil.

Functionality Comparison of CaPO4, Lipofectamine 2000 or TransIT-Lenti Generated Lentivirus.

Comparison of CaPO4, Lipofectamine® 2000 or TransIT®-Lenti Generated Lentivirus.  HIV CMVeGFP Virus was produced in HEK 293FT cells using either CaPO4, Lipofectamine® 2000 or TransIT®-Lenti Transfection Reagent per the manufacturer’s protocol. Lentivirus was collected 48 hours post-transfection and concentrated by prolonged centrifugation at 9,000 x g. HT1080 cells were infected with a 1:100 or 1:1000 dilution of each concentrated lentivirus. Images (above) were captured 48 hours post-transduction.

Data courtesy of Jeremy Coffin, University of Iowa Viral Vector Core

Product Resources

COA Lookup

Download Certificates of Analysis

COO Lookup

Download Certificate of Origin

select

Feedback

This product does not yet have related testimonials.

Watch Us On YouTube
Close