TransIT®-Lenti Transfection Reagent

Ideal for Recombinant Lentivirus Production

  Product No. Quantity Price Add to Cart
TransIT®-Lenti Transfection Reagent
  MIR 6603 0.3 ml $120.00
  MIR 6604 0.75 ml $300.00
  MIR 6600 1.5 ml $475.00
  MIR 6605 5 x 1.5 ml $2,066.00
  MIR 6606 10 x 1.5 ml $3,800.00
TransduceIT™ Transduction Reagent
  MIR 6620 1 ml $25.00

Product Overview

TransIT®-Lenti Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent HEK 293T cell types and increase recombinant lentivirus production.

  • High Performance – Provide up to eight-fold higher functional titers
  • Simple Protocol – No media change required, single harvest
  • Animal Origin Free  Regulatory friendly
Glossary of Lentivirus Terms Click to expand
Overview of Recombinant Lentivirus ProductionClick to expand

Figures and Data

High Functional Titers with TransIT-Lenti Transfection Reagent

High Functional Titers with TransIT®-Lenti Transfection Reagent. Adherent 293T/17 cells were transfected in a 6-well plate with pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION® (1:1 ratio, 2 µg/well) with the following reagents: TransIT®-Lenti (3:1, vol:wt), Lipofectamine® 2000 (3:1), Lipofectamine® 3000 (3:1:1), 25 kDa PEI (6:1), or CaPO4 precipitation (4 µg pDNA/well). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

High Transduction Efficiency in Neuron Cells with Unconcentrated Lentivirus Using TransIT-Lenti

High Transduction Efficiency with Unconcentrated Lentivirus Using TransIT®-Lenti. (A) Lentivirus was produced with the TransIT®-Lenti Transfection Reagent (3:1, vol:wt) or Lipofectamine® 2000 using the MISSION® vectors (pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION®). The supernatant was harvested, filtered (0.45 µm), and frozen. Lentivirus transductions were performed 5 days post-plating with iCell® Motor Neurons (Cellular Dynamics International). For bothTransIT-Lenti and Lipofectamine® 2000, one microliter of unconcentrated supernatant was added per well of a 96-well plate. GFP efficiency was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent the SEM of duplicate wells. (B) iCell® Motor Neurons were plated in Ibidy 35mm dishes and transduced withlentivirus produced using the TransIT®-Lenti Transfection Reagent and MISSION® vectors. Images were captured at 72 hours post-transduction with a Zeiss Axiovert S100 inverted fluorescence microscope using a 63X objective under oil.

Functionality Comparison of CaPO4, Lipofectamine 2000 or TransIT-Lenti Generated Lentivirus.

Comparison of CaPO4, Lipofectamine® 2000 or TransIT®-Lenti Generated Lentivirus.  HIV CMVeGFP Virus was produced in HEK 293FT cells using either CaPO4, Lipofectamine® 2000 or TransIT®-Lenti Transfection Reagent per the manufacturer’s protocol. Lentivirus was collected 48 hours post-transfection and concentrated by prolonged centrifugation at 9,000 x g. HT1080 cells were infected with a 1:100 or 1:1000 dilution of each concentrated lentivirus. Images (above) were captured 48 hours post-transduction.

Data courtesy of Jeremy Coffin, University of Iowa Viral Vector Core

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