CHOgro® Expression System

High titer transient transfection system for suspension CHO cells

CHOgro® Expression System ComponentsClick to expand
  Product No. Quantity Price Add to Cart
CHOgro® Expression System
  MIR 6260 1 Kit $536.00
TransIT-PRO® Transfection Reagent
  MIR 5740 1 ml $365.00
CHOgro® Expression Medium
  MIR 6200 1 Liter $102.00
CHOgro® Complex Formation Solution
  MIR 6210 100 ml $26.00
Poloxamer 188 Solution, 10%
  MIR 6230 100 ml $26.00
L-Glutamine Solution, 200 mM
  MIR 6240 100 ml $26.00

Product Overview

Ideal for Biotherapeutic Protein Production in Suspension CHO cells

The CHOgro® Expression System was developed through systematic optimization of transfection protocol parameters including: cell density, transfection reagent, media formulation and culture temperature (learn more about how this system was developed). With the CHOgro® Expression System, high protein titers can now be achieved in suspension CHO cells through high density transient transfection. Salient features include:

  • Efficient – Enables high protein titers with simple workflow
  • Convenient – Quick adaptation to CHO cell lineages
  • Optimized – High density growth with minimal cell clumping post transfection
  • Worry-free – No commercial license required; animal origin free

Experimental Data | Frequently Asked Questions | Using PEI? Realize Cost Savings | TransIT-PRO® for High Yield Protein Production

CHOgro® Expression Medium now available in 10 Liter quantities! (powder or liquid format)

 

Figures and Data

Titers of Five Different Antibody Vector Constructs Using the CHOgro Expression System

Titers of Five Different Antibody Vector Constructs Using the CHOgro® Expression System. Five different antibody constructs were produced by transient transfection using TransIT-PRO® at a 1:1 reagent:DNA ratio. Transfections were performed using 1 µg plasmid DNA per milliliter of culture and a cell density of 2 x 106 cells/ml at the time of transfection.  FreeStyle™ CHO-S cells were cultured in CHOgro® Expression Medium and seeded into 30 ml media in 125 ml shake flasks (Thomson) for transfection. Day 11 supernatants were clarified and analyzed using a human IgG ELISA (ZeptoMetrix). Error bars represent the standard deviation of triplicate technical replicates.

CHOgro Expression System Enables Broad Scalability, 1000-fold

CHOgro® Expression System Enables Broad Scalability, 1000-fold. Human IgG1 was produced by transient transfection with the TransIT-PRO® Transfection Reagent and 1 µg plasmid DNA per milliliter of culture at a 1:1 reagent:DNA ratio. Cells were transfected at a density of 2 x 106 cells/ml in CHOgro® Expression Medium on an orbital shaker at the following volumes/culture vessels: 2.5 ml/non-tissue culture treated 6-well dish, 25 ml/125 ml Thomson flask, 100 ml/250 ml Thomson flask, 500 ml/1.6 L Thomson flask, 2500 ml/5 L Thomson flask. At twenty-four hours post-transfection all cultures were moved to 32°C for the remainder of the experiment. Antibody levels  were analyzed from day 7 clarified supernatants using a human IgG ELISA (Zeptometrix). All values are normalized to the 25 ml volume sample and error bars represent the standard error of the mean of triplicate technical replicates.

Higher Cell Densities Leads to Higher Titers Using the CHOgro Expression System

Higher Cell Densities Leads to Higher Titers Using the CHOgro® Expression System. Human IgG1 was produced by transient transfection using TransIT-PRO® (1:1) or FreeStyle™ MAX (1.25:1.25) transfection reagents according to the manufacturers' protocol (reagent:DNA ratio, volume:weight). Transfections were performed using 1 µg plasmid DNA per milliliter of culture and cell densities of 1, 2 or 4 x 106 cells/ml at the time of transfection. FreeStyle™ CHO-S cells were cultured in CHOgro® Expression Medium (red bars) or FreeStyle™ CHO Expression Medium (blue bars) and plated into non-treated 6-well plates (2 ml/well) for transfection. Day 6 supernatants were clarified and analyzed using a human IgG ELISA (ZeptoMetrix). Error bars represent the standard deviation of triplicate technical replicates.

Product Resources

Specifications

Storage Conditions:
All Configurations: Multiple storage conditions - see individual bottles for specific recommendations

Product Guarantee:
All Configurations: As labeled on the product components

Technical Product Literature


Additional Resources

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