NEW! CHOgro® Expression Media and Supplements

High density growth and quick adaptation of suspension CHO cells

  Product No. Quantity Price Add to Cart
CHOgro® Expression Medium
  MIR 6200 1 Liter $102.00
  MIR 6202 10 Liter Polybag $969.00
CHOgro® Expression Medium - Dry Powder
  MIR 6201 Prepares 10 Liters $867.00
CHOgro® Complex Formation Solution
  MIR 6210 100 ml $26.00
Poloxamer 188 Solution, 10%
  MIR 6230 100 ml $26.00
L-Glutamine Solution, 200 mM
  MIR 6240 100 ml $26.00

Product Overview

Ideal for Biotherapeutic Protein Production in Suspension CHO cells

CHOgro® Expression Medium is a chemically defined, hydrolysate-free and animal-origin-free medium manufactured under cGMP conditions in an ISO-compliant facility. CHOgro® is formulated to provide high density cell growth, and many suspension CHO cells (e.g. Freestyle CHO-S) can easily and quickly grow in CHOgro® Expression Medium with minimal adaptation. CHOgro® Expression Medium requires supplementation with L-Glutamine and Poloxamer 188 before use.

CHOgro® Complex Formation Solution is an animal-origin-free solution specifically formulated for optimal transfection complex formation in conjunction with CHOgro® Expression Media and TransIT-PRO® Transfection Reagent to provide a completely animal-origin-free expression system.

  • High Density Growth – CHO-S cells grow > 12 x 106cells/ml
  • Quick Adaptation – CHO-S cells double within 24hr of complete media change from FreeStyle™ Expression Media
  • No Cell Clumping Post-Transfection – Obtain accurate cell counts and high viability
  • Quality – CHOgro® Medium and Complex Formation Solution are manufactured under cGMP conditions in an ISO-compliant facility
  • Regulatory Friendly – ALL components are animal origin free

Development and Optimization of CHOgro® Transient Expression Technologies for High Titer Antibody Production in Suspension CHO cells

NEW! CHOgro® Expression Medium now available in 10 Liter quantities! (powder or liquid format)

Figures and Data

CHOgro Expression Medium Yields Multi-fold Increases in Antibody Titer

CHOgro® Expression Medium Yields Multi-fold Increases in Antibody Titer. Human IgG1 was produced by transient transfection using TransIT-PRO® (1:1), FreeStyle™ MAX (1.25:1.25)  or 25kDa linear PEI (6:1) transfection reagents according to the manufacturers’ or published protocol (reagent:DNA ratio). Transfections were performed using 1 µg plasmid DNA per milliliter of culture and cell densities of 2 x 106 cells/ml or 1x 106 cells/ml  for the CHOgro® Expression Medium (red bars) or FreeStyle™  Expression Medium (blue bars), respectively, at the time of transfection.  FreeStyle™ CHO-S cells were cultured in CHOgro® Expression Medium or FreeStyle™ CHO Expression Medium  and plated into non-treated 6-well plates (2ml/well) for transfection. Antibody levels were also analyzed from day 6 clarified supernatants using a human IgG ELISA (ZeptoMetrix). Error bars represent the standard deviation of triplicate technical replicates.

Suspension CHO Cells Grow to High Density in the CHOgro Expression Medium

Suspension CHO Cells Grow to High Density in the CHOgro® Expression Medium. Triplicate flasks of FreeStyle™ CHO-S cells were seeded in CHOgro® Expression Medium (red line) or FreeStyle™ CHO Expression Medium (blue line) at cell density of 0.5 x 106 cells/ml, 40 ml per 125 ml shake flask (Thomson). Cell counts (solid line) and viability (propidium iodide staining, dotted line) were measured daily using a Guava® easyCyte 5HT flow cytometer (EMD Millipore).  Error bars represent the standard deviation of three readings of biological triplicates.

CHOgro Media Exchange Leads to Higher Protein Production

CHOgro® Media Exchange Leads to Higher Protein Production. FreeStyle™ CHO-S cells were cultured in FreeStyle™ CHO Expression Medium or CHOgro® Expression Medium.  Twenty four hours prior to transfection a subset of the cells grown in FreeStyle™ CHO Expression Medium were spun down and exchanged with 100% fresh CHOgro® Expression Medium.  The cells were allowed to grow and adapt for 24 hours prior to transfection with FreeStyle™ MAX (1.25:1.25) or TransIT-PRO® (1:1)  transfection reagents according to the manufacturers’ protocol (reagent:DNA ratio) and a human IgG1 encoding construct. Transfections were performed using 1 µg plasmid DNA per milliliter of culture and cell densities of 1 x 106 cells/ml for cells transfected with FreeStyle™ Max and 2 x 106 cells/ml for cells transfected with TransIT-PRO®. All cells were plated into non-treated 6-well plates (2ml/well) for transfection. (A) Workflow schematic of media exchange of CHO-S cells from FreeStyle™ CHO Expression Medium to CHOgro® Expression Medium (black arrow) or the normal CHOgro® Expression System (red arrow) (B) Day 6 supernatants were clarified and analyzed using a human IgG ELISA (ZeptoMetrix). Data is normalized to the complete CHOgro® Expression System (red bar).  Error bars represent the standard deviation of triplicate technical replicates.

Product Resources

Specifications

Storage Conditions:
CHOgro® Expression Medium: Store at 4°C
CHOgro® Expression Medium - Dry Powder: Store at 4°C
CHOgro® Complex Formation Solution: Store at 4°C
Poloxamer 188 Solution, 10%: Store at room temperature
L-Glutamine Solution, 200 mM: Store at -20°C

Product Guarantee:
All Configurations: As labeled on the product

Technical Product Literature


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