NOTICE: The Label IT µArray Labeling Kits (MIR 8010, MIR 8050, MIR 8105, MIR 8125, MIR 8205, MIR 8225, MIR 8710, MIR 8750, MIR 8810, MIR 8850) are discontinued. Please contact the technical services team with questions (techsupport@mirusbio.com) or our customer service to purchase any existing inventory (sales@mirusbio.com).

Label IT® µArray Biotin Labeling Kit

Optimized labeling kit designed for single-channel microarray applications using Biotin

One or more products on this page are on back order or are discontinued. Please contact techsupport@mirusbio.com if you have any questions.
  Product No. Quantity Price Add to Cart
Label IT® µArray Labeling Kit, Biotin
  MIR 8010 10 reactions
This product has been discontinued and is no longer available.
$238.00 
  MIR 8050 50 reactions
This product has been discontinued and is no longer available.
$1,095.00 

Note: Each reaction labels 1 µg of nucleic acid.

Product Overview

  • One-step Chemical Method – Easily and consistently achieve optimal labeling densities for microarray applications
  • Sensitive Detection – Confidently detect rare transcripts and small changes in gene expression
  • Versatile Labeling  Suitable for labeling mRNA, cDNA and cRNA templates that are used for single-channel expression profiling analyses

Kit Components:
Each Kit contains Label IT® uArray Biotin Reagent, Reconstitution Solution, 10X Labeling Buffer M, Reagent D1, Buffer N1, 0.5M EDTA and 5X Fragmentation Buffer. Biotin detection reagents are not included.

Figures and Data

Label IT uArray Biotin Labeling Kit

Label IT® µArray Biotin Labeling Kit. The Label IT® biotin reagent is composed of three regions: the biotin label (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT® biotin reagent to any reactive heteroatom within cDNA or rRNA molecules. The covalent attachment of the Label IT® reagents to cDNA or cRNA does not alter their structure or downstream hybridization performance, and thus, once labeled, the cDNA or cRNA samples can be hybridized to gene expression microarrays, detected and analyzed to determine the gene expression profile.

Label IT uArray Biotin Kit Labels any Nucleic Acid Sample

Label IT® µArray Biotin Kit Labels any Nucleic Acid Sample. The Label IT® µArray Biotin Reagent was used to label mRNA directly for hybridization to duplicate double-stranded cDNA arrays (top), or cDNA and cRNA samples for hybridization to sense-strand oligo arrays (middle, bottom). Labeled samples were detected using a Streptavidin-Cy®3 conjugate.

Gene Expression Profiles of Labeled mRNA and cDNA Samples

Gene Expression Profiles of Labeled mRNA and cDNA Samples. HeLa mRNA and cDNA samples, prepared with the Label IT® µArray Biotin Kit generate the same gene expression profiles as cDNA samples labeled with reverse transcriptase incorporation of biotinylated nucleotides. Slightly different quantification of some genes (e.g. A04, C09) with labeled mRNA compared to either method of labeling cDNA may reflect enzymatic replication bias inherent with cDNA sample preparation, and demonstrate the advantage of directly labeling and hybridizing mRNA to microarrays. Relative gene expression values for each labeling method were generated from the average of multiple replicate features from six technical replicate arrays using the same HeLa total RNA sample (data from each microarray hybridization were normalized before averaging).

Product Resources

Specifications

Storage Conditions:
All Configurations: Store the Label IT® Reagent at -20°C both as dried pellets and after reconstitution. Store all other supplied reagents at 4°C or -20°C.

Product Guarantee:
All Configurations: 1 year (6 months after reconstitution)

Technical Product Literature


Additional Resources

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