NOTE: Each kit contains sufficient reagents to perform at least 50 transfections in 6-well plates.
Intracellular Localization and Expression of Labeled Plasmid DNA Using Label IT® Tracker™ Nucleic Acid Labeling Kits Nucleic Acid Labeling and Delivery Tools for Neurobiology
Kit Components: Each Kit contains Label IT® Tracker™ Reagent, Label IT® Reconstitution Solution, and 10X Labeling Buffer.
Label IT® Tracker™ Kits. The Label IT® Tracker™ labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with plasmid DNA and the reactive alkylating group (blue) that covalently attaches the Label IT® reagents to any reactive heteroatom within the plasmid. Attachment of the Label IT® Reagent to plasmids does not alter their structure or affect downstream gene expression, and as such, the same labeled plasmid can be both visualized after transfection by fluorescence microscopy and employed to express the gene of interest on the plasmid.
Tracking of Plasmid Localization and Expression. COS-7 cells transfected with Label IT® Tracker™ Cy®5 (blue) labeled pEYFP-nuc and TransIT®-LT1 Reagent in serum containing media. Images were acquired at 3, 8, and 20 hours post-transfection. The blue signal indicates the cellular localization of the labeled plasmid, the green signal indicates cellular autofluorescence, and the yellow signal is the expression of the nuclear yellow fluorescent protein (YFP) reporter.
Label IT® Excitation and Emission Spectra Technical Report: Delivery Tools for Neurobiology Technical Report: Intracellular Localization and Expression
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