Label IT® Tracker™ Intracellular Nucleic Acid Localization Kits

Efficient, direct labeling of plasmid DNA for in vitro and in vivo tracking experiments

CUSTOMER NOTICE
To provide a better customer experience, Mirus Bio has changed the Label IT® reagent cap to a high-profile design with a red cap insert to make it easier to handle and to identify. Please contact techsupport@mirusbio.com if you have any questions.

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NOTE: Each kit contains sufficient reagents to perform at least 50 transfections in 6-well plates.

Technical Reports

Intracellular Localization and Expression of Labeled Plasmid DNA Using Label IT® Tracker™ Nucleic Acid Labeling Kits
Nucleic Acid Labeling and Delivery Tools for Neurobiology

  • Versatile Labeling – Efficiently label and visualize the DNA of your choice
  • One-step Chemical Method – Easily and consistently control the labeling reactions
  • High Efficiency Labeling – Optimal visualization of DNA in cells
  • Superior Tracking – Monitor both subcellular localization and reporter transgene expression following transfection of labeled (plasmid) DNA

Kit Components:
Each Kit contains Label IT® Tracker™ Reagent, Label IT® Reconstitution Solution, and 10X Labeling Buffer.

Figures and Data

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Label IT Tracker Kits

Label IT® Tracker™ Kits. The Label IT® Tracker™ labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with plasmid DNA and the reactive alkylating group (blue) that covalently attaches the Label IT® reagents to any reactive heteroatom within the plasmid. Attachment of the Label IT® Reagent to plasmids does not alter their structure or affect downstream gene expression, and as such, the same labeled plasmid can be both visualized after transfection by fluorescence microscopy and employed to express the gene of interest on the plasmid.

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Tracking of Plasmid Localization and Expression

Tracking of Plasmid Localization and Expression. COS-7 cells transfected with Label IT® Tracker™ Cy®5 (blue) labeled pEYFP-nuc and TransIT®-LT1 Reagent in serum containing media. Images were acquired at 3, 8, and 20 hours post-transfection. The blue signal indicates the cellular localization of the labeled plasmid, the green signal indicates cellular autofluorescence, and the yellow signal is the expression of the nuclear yellow fluorescent protein (YFP) reporter.

Resources

Specifications

Storage Conditions:
All Configurations: Store the Label IT® Reagent at -20°C in both its dried pellet and reconstituted form. Store all other supplied reagents at -20°C. Warm kit components to room temperature and quick spin before each use.

Product Guarantee:
All Configurations: 1 year (6 months after reconstitution)

Technical Product Literature


Additional Resources

COA Lookup

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Testimonials

"Excellent technical support, they are very knowledgeable."

Review published in Select Science

Questions?

Contact Technical Support
8:30AM - 4:00PM CST, M-F
EMAIL - techsupport@mirusbio.com
PHONE - 888.530.0801