Close
Email techsupport@mirusbio.com sales@mirusbio.com
Phone 888.530.0801 Customer Service: 8:00AM - 4:00PM CST, M-F Technical Support: 8:30AM - 4:00PM CST, M-F Order Online 24 Hours a Day
Facebook Twitter Youtube
Product Recommendations From Reagent Agent
To inquire about bulk pricing, please call 888-530-0801 International inquiries please call +1-608-441-2852 View Shipping Information
* Additional freight charges may apply for shipments heavier than 10 lbs. Please contact customer service to verify shipping charges: 888-530-0801.
Choose a Product:
Select a Size:
Catalog number selected: -
-
Please contact customer service via sales@mirusbio.com or +1-608-441-2852 to request a quote for this product or complete the short form below and a customer service representative will contact you.
NOTE: Each kit contains sufficient reagents to perform at least 50 transfections in 6-well plates.
Intracellular Localization and Expression of Labeled Plasmid DNA Using Label IT® Tracker™ Nucleic Acid Labeling Kits Nucleic Acid Labeling and Delivery Tools for Neurobiology
Kit Components: Each Kit contains Label IT® Tracker™ Reagent, Label IT® Reconstitution Solution, and 10X Labeling Buffer.
Label IT® Tracker™ Kits. The Label IT® Tracker™ labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with plasmid DNA and the reactive alkylating group (blue) that covalently attaches the Label IT® reagents to any reactive heteroatom within the plasmid. Attachment of the Label IT® Reagent to plasmids does not alter their structure or affect downstream gene expression, and as such, the same labeled plasmid can be both visualized after transfection by fluorescence microscopy and employed to express the gene of interest on the plasmid.
Tracking of Plasmid Localization and Expression. COS-7 cells transfected with Label IT® Tracker™ Cy®5 (blue) labeled pEYFP-nuc and TransIT®-LT1 Reagent in serum containing media. Images were acquired at 3, 8, and 20 hours post-transfection. The blue signal indicates the cellular localization of the labeled plasmid, the green signal indicates cellular autofluorescence, and the yellow signal is the expression of the nuclear yellow fluorescent protein (YFP) reporter.
Technical Report: Delivery Tools for Neurobiology Technical Report: Intracellular Localization and Expression
Download Certificates of Analysis
For successful COA look up, please use the following tips to locate the appropriate lot number for the respective product categories. If your COA search is not successful using these tips OR you purchased Mirus products before 2011, please request a COA by emailing techsupport@mirusbio.com.
"Excellent technical support, they are very knowledgeable."
He Song Sun University of Toronto Scarborough - Cell and Molecular Biology
Review published in Select Science
Contact Technical Support 8:30AM - 4:00PM CST, M-F EMAIL - techsupport@mirusbio.com PHONE - 888.530.0801