TransIT®-QR Delivery Solution and Starter Kit

A nontoxic, "Quick Recovery" in vivo delivery solution that delivers DNA or siRNA to the livers of laboratory mice using hydrodynamic tail vein injection.

  Product No. Quantity Price Add to Cart
TransIT®-QR Starter Kit
  MIR 5210 10 injections $117.00
TransIT®-QR Delivery Solution
  MIR 5240 40 injections $173.00

Product Overview

  • Naked Nucleic Acid Delivery – Achieve higher levels of gene expression than compared to polylysine and PEI methods
  • Versatile Platform – for DNA or siRNA delivery using hydrodynamic tail vein injection to the mouse strain of your choice
  • Low Toxicity – Minimized loss of cardiac output compared to saline injections, which allows for quick recovery (within minutes) of the mouse post-injection

Starter Kit Components:
Each TransIT®-QR Starter Kit contains the TransIT®-QR Delivery Solution, 10 syringes, needles and alcohol swabs, and a conical plastic tube to restrain the mouse while tail vein injection is performed. The TransIT®-QR Delivery Solution is also sold separately as indicated above.

Figures and Data

TransIT-QR Solution Effectively Delivers the Label IT Cy3 RNAi Delivery Control to Hepatocytes

TransIT®-QR Delivery Solution Effectively Delivers the Label IT® Cy®3 RNAi Delivery Control to Hepatocytes. TransIT®-QR Delivery Solution was used to deliver 25 µg of Label IT® Cy®3 RNAi Delivery Control (red) to a mouse using hydrodynamic delivery via the tail vein. Forty-five minutes post-injection the liver was harvested and sections were fixed then counterstained to stain the nuclei (blue) and to stain the actin (green).

Achieve Low Toxicity Using the TransIT-QR Delivery Solution

The TransIT®-QR Solution Minimizes the Loss of Cardiac Output Compared to Injections Performed Using Normal Saline. Mice were anesthetized, connected to an arterial blood pressure monitor, and injected with either TransIT®-QR (Quick Recovery) Delivery Solution (red) or normal saline (blue) using the hydrodynamic tail vein injection procedure. The relative cardiac output was estimated by multiplying the heart rate by the pulse pressure (difference between systolic and diastolic pressures) and scaled to the pre-injection cardiac output.

Knockdown in Multiple Organs After Co-delivery of siRNA and Target Plasmids

Reporter Gene Knockdown in Multiple Organs After Co-delivery of siRNA and Reporter Plasmids. Mice were injected with 10 µg of plasmid encoding firefly luciferase, 1 µg of plasmid encoding Renilla luciferase, 5 µg of siRNA targeted against firefly luciferase, and 5 µg of control siRNA using the TransIT®-QR Delivery Solution. Twenty-four hours later, the samples were assayed for firefly and Renilla luciferase activity. The level of firefly luciferase expression was normalized to Renilla activity and compared to numbers obtained using the control siRNA. Lewis et al., Nature Genetics 32: 107-8, 2002.

NOTE: Following gene delivery via a tail vein injection, the highest level of transgene expression is found in the liver, with reduced levels of expression found in the spleen, lung, heart and kidneys. siRNA delivery to these organs is not sufficient to achieve appreciable endogenous targeted gene knockdown.

Product Resources

Specifications

Storage Conditions:
All Configurations: Store at 4°C

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


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