TransIT®-EE Delivery Solution and Starter Kit

An "Enhanced Expression" in vivo delivery solution that delivers DNA to the livers of laboratory mice using hydrodynamic tail vein injection

  Product No. Quantity Price Add to Cart
TransIT®-EE Starter Kit
  MIR 5310 10 injections $131.00
TransIT®-EE Delivery Solution
  MIR 5340 40 injections $229.00

Product Overview

  • Naked Nucleic Acid Delivery – Achieve increased levels of gene expression than compared to TransIT®-QR Delivery Solution, TransIT- In Vivo Gene Delivery System, polylysine and PEI methods
  • Versatile Platform – Suitable for DNA delivery using hydrodynamic tail vein injection to the mouse strain of your choice

Starter Kit Components:
Each starter kit contains the TransIT®-EE Delivery Solution, 10 syringes, needles and alcohol swabs; and a conical plastic tube to restrain the mouse while tail vein injection is performed. The TransIT®-EE Delivery Solution is also sold separately as indicated above.

Figures and Data

TransIT-EE Solution Leads to Enhanced Luciferase Expression in the Liver

TransIT®-EE Solution Leads to Enhanced Luciferase Expression in the Liver. TransIT®-QR or TransIT®-EE Delivery Solutions were used to deliver 10 µg of a luciferase expression vector to mice using the hydrodynamic delivery tail vein injection procedure. Twenty-four hours post-injection the livers were harvested and assayed for luciferase activity.

TransIT-EE Solution Leads to Enhanced Luciferase Expression in a Variety of Organs

TransIT®-EE Solution Leads to Enhanced Luciferase Expression in a Variety of Organs. TransIT®-QR or TransIT®-EE Delivery Solutions were used to deliver 10 µg of pCI-Luc (CMV promoter-driven luciferase expression vector) to mice using the hydrodynamic tail vein injection procedure. Twenty-four hours post-injection the organs were harvested and assayed for luciferase activity.

NOTE: Following gene delivery via a tail vein injection, the highest level of transgene expression is found in the liver, with reduced levels of expression found in the spleen, lung, heart and kidneys.

Product Resources

Specifications

Storage Conditions:
TransIT®-EE Starter Kit: Store at 4°C
TransIT®-EE Delivery Solution: Store at 4°C

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


Additional Resources

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