TransIT®-EE Delivery Solution

An "Enhanced Expression" in vivo delivery solution that delivers DNA to the livers of laboratory mice using hydrodynamic tail vein injection

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MIR 534040 injections

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  • Naked Nucleic Acid Delivery– Achieve increased levels of gene expression than compared to TransIT®-QR Delivery Solution, TransIT-In Vivo Gene Delivery System, polylysine and PEI methods
  • Versatile Platform – Suitable for DNA delivery using hydrodynamic tail vein injection to the mouse strain of your choice

Figures and Data

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TransIT-EE Solution Leads to Enhanced Luciferase Expression in the Liver

TransIT®-EE Solution Leads to Enhanced Luciferase Expression in the Liver. TransIT®-QR or TransIT®-EE Delivery Solutions were used to deliver 10 µg of a luciferase expression vector to mice using the hydrodynamic delivery tail vein injection procedure. Twenty-four hours post-injection the livers were harvested and assayed for luciferase activity.

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TransIT-EE Solution Leads to Enhanced Luciferase Expression in a Variety of Organs

TransIT®-EE Solution Leads to Enhanced Luciferase Expression in a Variety of Organs. TransIT®-QR or TransIT®-EE Delivery Solutions were used to deliver 10 µg of pCI-Luc (CMV promoter-driven luciferase expression vector) to mice using the hydrodynamic tail vein injection procedure. Twenty-four hours post-injection the organs were harvested and assayed for luciferase activity.

NOTE: Following gene delivery via a tail vein injection, the highest level of transgene expression is found in the liver, with reduced levels of expression found in the spleen, lung, heart and kidneys.

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Specifications

Storage Conditions:
All Configurations: Store at 4°C

Product Guarantee:
All Configurations: 1 year

Usage Statement:
All Configurations: For Research Use Only.

Animal Origin Statement:
All Configurations: This product is animal origin free.

Technical Product Literature


Additional Resources

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Testimonials

Excellent technical support, they are very knowledgeable.
He Song Sun
University of Toronto Scarborough - Cell and Molecular Biology