pLIVE® In Vivo Expression and Reporter Vectors

A vector system designed to achieve long term expression in the livers of laboratory mice

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  • Sustained Gene Expression – Achieve long term liver gene expression in the mouse liver through 8 months post-injection
  • Versatile Platform – Available with positive control vectors expressing either LacZ or SEAP

Maps

pLIVE Map 1

pLIVE Map 2

pLIVE Map 3

 

Sequences

The pLIVE® Vector

pLIVE Sequence
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The pLIVE®-LacZ Vector

pLIVE Sequence
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The pLIVE®-SEAP Vector

pLIVE Sequence
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Purchase of this product conveys a license for use of this product only for research by the purchaser in not-for-profit institutions. The purchaser may only transfer vectors modified with sequences for protein expression to researchers in not-for-profit institutions who agree to be bound by this license. This license does not include the right to perform research at or on behalf of a for-profit entity, the right to transfer unmodified vectors to others or the right to use the product for any commercial purpose (e.g. therapeutics, manufacturing, diagnostics or sale or fee-for-service use of animal models). Prospective users at for-profit institutions or those who wish to perform research on behalf of for-profit institutions or any other commercial purpose must contact Mirus Bio LLC for a commercial license. If you require a commercial license to use this product and you decide not to take a license, please return the unopened product to Mirus Bio LLC for a full refund.

Figures and Data

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The Promoter in the pLIVE Vector Outperforms the CMV Promoter for Long-term Liver Expression

The Promoter in the pLIVE® Vector Outperforms the CMV Promoter for Long-term Liver Expression. The pLIVE®-SEAP Vector (20 µg) and pCMV-SEAP (10 µg, CMV promoter-driven SEAP vector) were each delivered to four ICR mice using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR 5240). At the indicated days post-injection, serum from each mouse was collected and the level of SEAP activity present was determined using the Phospha-Light™ SEAP Assay Kit (Applied Biosystems). The average SEAP activity from each group is presented.

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Long-term Expression of Human Placental Secreted Alkaline Phosphatase (SEAP) in Mice after Hydrodynamic Delivery

Long-term Expression of Human Placental Secreted Alkaline Phosphatase (SEAP) in Mice after Hydrodynamic Delivery of the pLIVE®-SEAP Vector. The pLIVE®-SEAP Vector was delivered to three C57Bl/6 mice using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR 5240). At the indicated times post-injection, serum from each mouse was collected and the level of SEAP activity was determined using the Phospha-Light™ SEAP Assay Kit (Applied Biosystems). Average SEAP activity at each time point is presented.

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Strong Liver Expression of ß-galactosidase from pLIVE-lacZ Vector after Hydrodynamic Tail Vein Injection

Strong Liver Expression of ß-galactosidase from pLIVE®-lacZ Vector after Hydrodynamic Tail Vein Injection. The pLIVE®-lacZ Vector (Panel A) was delivered to an ICR mouse using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR 5240). At 24 hours post-injection, the liver was harvested, sectioned and stained with X-gal using the Beta-Gal Staining Kit (MIR 2600) to demonstrate ß-galactosidase activity (blue cells). The cells were then counterstained with hematoxylin and eosin Y to stain the nuclei and cytoplasms, respectively. The control mouse (lacZ negative) in Panel B was stained in parallel to Panel A and contains no detectable ß-galactosidase activity.

Resources

Specifications

Storage Conditions:
All Configurations: Store vector DNA at -20°C. Prior to use, thaw vector DNA at room temperature, vortex, and quick spin in a microcentrifuge.

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


Additional Resources

COA Lookup

Download Certificates of Analysis

Testimonials

"Excellent technical support, they are very knowledgeable."

Review published in Select Science

Questions?

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PHONE - 888.530.0801