pLIVE® In Vivo Expression and Reporter Vectors

A vector system designed to achieve long term expression in the livers of laboratory mice

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MIR 542020 µg

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  • Sustained Gene Expression – Achieve long term liver gene expression in the mouse liver through 8 months post-injection
  • Versatile Platform – Available with positive control vectors expressing either LacZ or SEAP

Maps

pLIVE Map 1

pLIVE Map 2

pLIVE Map 3

 

Sequences

The pLIVE® Vector

pLIVE Sequence
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The pLIVE®-LacZ Vector

pLIVE Sequence
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The pLIVE®-SEAP Vector

pLIVE Sequence
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Purchase of this product conveys a license for use of this product only for research by the purchaser in not-for-profit institutions. The purchaser may only transfer vectors modified with sequences for protein expression to researchers in not-for-profit institutions who agree to be bound by this license. This license does not include the right to perform research at or on behalf of a for-profit entity, the right to transfer unmodified vectors to others or the right to use the product for any commercial purpose (e.g. therapeutics, manufacturing, diagnostics or sale or fee-for-service use of animal models). Prospective users at for-profit institutions or those who wish to perform research on behalf of for-profit institutions or any other commercial purpose must contact Mirus Bio LLC for a commercial license. If you require a commercial license to use this product and you decide not to take a license, please return the unopened product to Mirus Bio LLC for a full refund.

Figures and Data

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The Promoter in the pLIVE Vector Outperforms the CMV Promoter for Long-term Liver Expression

The Promoter in the pLIVE® Vector Outperforms the CMV Promoter for Long-term Liver Expression. The pLIVE®-SEAP Vector (20 µg) and pCMV-SEAP (10 µg, CMV promoter-driven SEAP vector) were each delivered to four ICR mice using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR 5240). At the indicated days post-injection, serum from each mouse was collected and the level of SEAP activity present was determined using the Phospha-Light™ SEAP Assay Kit (Applied Biosystems). The average SEAP activity from each group is presented.

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Long-term Expression of Human Placental Secreted Alkaline Phosphatase (SEAP) in Mice after Hydrodynamic Delivery

Long-term Expression of Human Placental Secreted Alkaline Phosphatase (SEAP) in Mice after Hydrodynamic Delivery of the pLIVE®-SEAP Vector. The pLIVE®-SEAP Vector was delivered to three C57Bl/6 mice using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR 5240). At the indicated times post-injection, serum from each mouse was collected and the level of SEAP activity was determined using the Phospha-Light™ SEAP Assay Kit (Applied Biosystems). Average SEAP activity at each time point is presented.

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Strong Liver Expression of ß-galactosidase from pLIVE-lacZ Vector after Hydrodynamic Tail Vein Injection

Strong Liver Expression of ß-galactosidase from pLIVE®-lacZ Vector after Hydrodynamic Tail Vein Injection. The pLIVE®-lacZ Vector (Panel A) was delivered to an ICR mouse using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR 5240). At 24 hours post-injection, the liver was harvested, sectioned and stained with X-gal to demonstrate ß-galactosidase activity (blue cells). The cells were then counterstained with hematoxylin and eosin Y to stain the nuclei and cytoplasms, respectively. The control mouse (lacZ negative) in Panel B was stained in parallel to Panel A and contains no detectable ß-galactosidase activity.

Resources

Specifications

Storage Conditions:
All Configurations: Store vector DNA at -20°C. Prior to use, thaw vector DNA at room temperature, vortex, and quick spin in a microcentrifuge.

Product Guarantee:
All Configurations: 1 year

Usage Statement:
All Configurations: For Research Use Only.

Animal Origin Statement:
All Configurations: This product is animal origin free.

Technical Product Literature


Additional Resources

COA Lookup

Download Certificates of Analysis

Testimonials

Excellent technical support, they are very knowledgeable.
He Song Sun
University of Toronto Scarborough - Cell and Molecular Biology