Mirus offers high quality cell culture grade selection antibiotics with the following features:
- Ease of use – Sterile filtered ready-to-use stock solutions for stable cell line generation
- High Purity – Greater than 90% purity determined by HPLC
- High Potency – In combination with high purity ensures better selection at optimal concentrations
Stable cell line generation allows for sustained expression of an exogenous gene which is commonly enriched and maintained through the use of selection antibiotics such as G418, Hygromycin B, Puromycin, etc. Resistance to these antibiotics is conferred through the expression of a positive selection gene: neomycin resistance (neo), hygromycin resistance (hph) and puromycin-N-acetyl-transferase (pac) genes, respectively.
Selection markers can be delivered using the same plasmid (in cis) that contains the gene of interest, or on a separate plasmid (in trans) that needs to be co-transfected with the plasmid containing the gene of interest. For a detailed protocol, please visit our Stable cell line generation web page.
Looking for cell culture antibiotics? Mirus also offers Cell Culture Antibiotics for prevention of contamination.