Phosphatase Inhibitor Cocktail

For inhibition of serine/threonine and protein tyrosine phosphatases

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Mirus Bio Phosphatase Inhibitor Cocktail provides the following features and benefits:

  • Convenient – Optimized formulation suitable for cell and tissue extracts
  • High Activity – Effectively protects phosphoproteins from phosphatases present in cell lysates
  • Broad Spectrum – Inhibits serine/threonine phosphatases and protein tyrosine phosphatases

Phosphorylation/dephosphorylation state serves as a molecular switch that controls diverse biological processes. The ability to preserve protein phosphorylation during cell lysis and protein purification is critical to studying the biological role of phosphorylation state on target molecules.

Mirus Phosphatase Inhibitor Cocktail contains an optimized combination of four broad spectrum phosphatase inhibitors (sodium fluoride, sodium orthovanadate, sodium pyrophosphate and β-glycerophosphate) that protect target proteins from dephosphorylation by serine/threonine phosphatases and protein tyrosine phosphatases.

Phosphatase Inhibitor Cocktail Components

Component Concentration Target
Sodium Fluoride 250 mM Acid Phosphatases
Sodium Orthovanadate 50 mM Protein Tyrosine Phosphatase
Alkaline Phosphatase
Sodium Pyrophosphate 50 mM PP1 and PP2A
β-Glycerophosphate 50 mM Serine/Threonine Phosphatases

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