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MiraCLEAN® Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per mg of DNA (EU/mg). The DNA was treated with the MiraCLEAN® Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.
Plasmid DNA Exhibits High Transfection Efficiency After Treatment with MiraCLEAN® Kit. COS-7 cells were transfected with 2 µg of plasmid DNA and 2µl of the TransIT®-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression.
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