MiraCLEAN® Endotoxin Removal Kit

A convenient and effective method for the removal of endotoxin from plasmid DNA preparations

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  • Effectively Removes Endotoxin from Plasmid Samples – Aids in the safety and efficiency of gene delivery research.
  • Compatible With In Vivo and In Vitro Applications – Removes harmful endotoxin that could cause decreased transfection efficiencies in vitro and inflammatory reactions in vivo .
  • Ease of Use – Simple phase separation protocol allows quick visualization of recovered nucleic acid preparations.

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MiraCLEAN Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations

MiraCLEAN® Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per mg of DNA (EU/mg). The DNA was treated with the MiraCLEAN® Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.

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Plasmid DNA Exhibits High Transfection Efficiency After Treatment with MiraCLEAN Kit

Plasmid DNA Exhibits High Transfection Efficiency After Treatment with MiraCLEAN® Kit. COS-7 cells were transfected with 2 µg of plasmid DNA and 2µl of the TransIT®-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression.

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Specifications

Storage Conditions:
All Configurations: Store at 4°C

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


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Testimonials

"Excellent technical support, they are very knowledgeable."

Review published in Select Science