MiraCLEAN® Endotoxin Removal Kit

A convenient and effective method for the removal of endotoxin from plasmid DNA preparations

  Product No. Quantity Price Add to Cart
MiraCLEAN® Endotoxin Removal Kit
  MIR 5910 for 10 mg DNA $164.00
  MIR 5900 for 100 mg DNA $484.00

Product Overview

  • Effectively Removes Endotoxin from Plasmid Samples – Aids in the safety and efficiency of gene delivery research.
  • Compatible With In Vivo and In Vitro Applications – Removes harmful endotoxin that could cause decreased transfection efficiencies in vitro and inflammatory reactions in vivo .
  • Ease of Use – Simple phase separation protocol allows quick visualization of recovered nucleic acid preparations.

Figures and Data

MiraCLEAN Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations

MiraCLEAN® Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per mg of DNA (EU/mg). The DNA was treated with the MiraCLEAN® Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.

Plasmid DNA Exhibits High Transfection Efficiency After Treatment with MiraCLEAN Kit

Plasmid DNA Exhibits High Transfection Efficiency After Treatment with MiraCLEAN® Kit. COS-7 cells were transfected with 2 µg of plasmid DNA and 2µl of the TransIT®-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression.

Product Resources

Specifications

Storage Conditions:
All Configurations: Store at 4°C

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


Additional Resources

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