Label IT® RNAi Delivery Controls

Fluorescently labeled RNAi duplexes ideal for in vitro or in vivo tracking

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  • Sensitive – Easily visualize transfected cells and assess delivery efficiency using fluorescent microscopy.
  • Inert – Not known to target any mammalian genes or cause off-target effects.
  • Compatible – Suitable for co-delivery experiments with functional siRNA.
  • Ease of Use – Supplied as a ready to use 10 µM stock solution with a 10X RNAi Dilution Buffer.

Label IT® Excitation and Emission Spectra

Figures and Data

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Label IT RNAi Delivery Controls Allow Quick Assessment of Delivery Efficiency For In Vitro Applications

Label IT® RNAi Delivery Controls Allow Quick Assessment of Delivery Efficiency For In Vitro Applications. HeLa cells were transfected in serum-containing media with (A) Label IT® Cy®3 RNAi Delivery Control (red) using the
TransIT-siQUEST® Transfection Reagent and (B) Label IT® Fluorescein RNAi Delivery Control (green) using the TransIT-TKO® Transfection Reagent. Twenty-four hours post-transfection, the cells were fixed, then counterstained to locate the nuclei (blue) and the actin (green).

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Visualization of the Label IT Cy3 RNAi Delivery Control in Liver Sections Following Tail Vein Injection

Visualization of the Label IT® Cy®3 RNAi Delivery Control in Liver Sections Following Tail Vein Injection. TransIT®-QR Hydrodynamic Delivery Solution was used to deliver 25 µg of Label IT® Cy®3 RNAi Delivery Control (red) to a mouse using hydrodynamic delivery via the tail vein. Forty-five minutes post-injection the liver was harvested. Sections were processed to stain the nuclei (blue) and to stain the actin (green).

Resources

Specifications

Storage Conditions:
All Configurations: Store at -20ºC, protected from exposure to light. Store the 10X RNAi Dilution Buffer at 4ºC.

Product Guarantee:
All Configurations: 1 year

Technical Product Literature


Additional Resources

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Testimonials

"Excellent technical support, they are very knowledgeable."

Review published in Select Science

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