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TransIT-VirusGEN Transfection Reagent – 0.75 mL

Proven Nucleic Acid Delivery for Large-Scale Virus Production

Description

TransIT-VirusGEN® Transfection Reagent is designed to enhance delivery of packaging and transfer plasmids to adherent and suspension HEK 293 cell types to increase recombinant adeno-associated virus (AAV) and lentivirus production.

Key benefits of TransIT-VirusGEN® Transfection Reagent include:

  • Performance – Efficient DNA delivery for production of high-titer viral vectors
  • Scalability – Efficient production from < 1 ml to large scale cultures
  • Flexibility – Compatible with different recombinant virus and cell culture systems
  • Animal Origin Free – Fully synthetic transfection reagent formulation

 

TransIT-VirusGEN® is ideal for academic research and biopharmaceutical discovery. Titers can be further increased with optimized enhancers included in the VirusGEN® AAV Transfection Kit and VirusGEN® LV Transfection Kit. For large-scale production of viral vectors, we recommend TransIT-VirusGEN® GMP.

SKU: MIR 6704

Supporting Data

 

TransIT-VirusGEN® Outperforms Competitor Reagents in Suspension and Adherent Lentivirus Cell Cultures Data Graph

TransIT-VirusGEN® Outperforms Competitor Reagents in Suspension & Adherent AAV Cell Cultures. Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio, 1.5 µg/ml, Agilent Technologies) with the following reagents: TransIT-VirusGEN® (2:1, vol:wt), Competitor B4 (3:1), Competitor B5 (3:3:1) or 25 kDa PEI (4:1, PolySciences). Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.

 

 

TransIT-VirusGEN Transfection Reagent Suspension Adherent Lentivirus Data

TransIT-VirusGEN® Outperforms Competitor Reagents in Suspension and Adherent Lentivirus Cell Cultures. Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION® (1:1 ratio, 1 µg/ml)  with the following reagents: TransIT-VirusGEN® (3:1, vol:wt), Competitor B4 (2:1 (A) or 3:1 (B)), Competitor B5 (2:2:1 (A) or 3:3:1 (B)) or 25 kDa PEI (4:1, Polysciences). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.

 

 

TransIT-VirusGEN Transfection Reagent Suspension Adherent AAV Scale-Up Data

TransIT-VirusGEN® Enables Broad Scalability (AAV). Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio, 1.5 total µg/ml, Agilent Technologies) using the TransIT-VirusGEN® Transfection Reagent (2:1 reagent-to-DNA ratio (vol:wt), Mirus) at the indicated volumes perculture vessel. Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction by flow cytometry. Functional titers were measured from virus dilutions with less than 20% GFP positive cells.  Error bars represent the standard deviation of triplicate wells, flasks, or dishes.

 

TransIT-VirusGEN Transfection Reagent Drobna Third Party Asset Technical Image

Higher Transfection Efficiency with TransIT-VirusGEN® Over PEI for Lentivirus Generation. HEK293T/17 cells were transfected with lentiviral packaging plasmids and either (a) control RFP transfer plasmid or (b) targeting SPdCas9-RFP transfer plasmids using either TransIT-VirusGEN® Transfection Reagent (3:1 reagent to DNA ratio; vol:wt) or 25 kDa linear PEI (3:1 reagent to DNA ratio; wt:wt). Brightfield and RFP Images were taken 17-22 hours post-transfection.

Data courtesy of Zuzana Drobna, PhD (Dr. Keung’s Lab), Department of Chemical and Biomolecular Engineering,  North Carolina State University

Resources

Specifications

Storage Conditions
TransIT-VirusGEN® Transfection Reagent: Store at -10 to -30°C
VirusGEN® AAV Transfection Kit: Multiple storage conditions – see individual bottles for specific recommendations
VirusGEN® LV Transfection Kit: Multiple storage conditions – see individual bottles for specific recommendations

Product Guarantee
TransIT-VirusGEN® Transfection Reagent: 12 months
VirusGEN® AAV Transfection Kit: 12 months for reagent, 6 months for complex formation solution and enhancer
VirusGEN® LV Transfection Kit: 12 months for reagent, 6 months for complex formation solution and enhancer

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

Technical Product Literature

Full Protocols
TransIT-VirusGEN® Full Transfection Protocol (PDF)
VirusGEN® AAV Transfection Kit Protocol (PDF)
VirusGEN® LV Transfection Kit Protocol (PDF)

Quick Reference Protocols
TransIT-VirusGEN® Quick Ref Protocol (PDF)
VirusGEN® AAV Transfection Kit QRP (PDF)
VirusGEN® LV Transfection Kit QRP (PDF)

SDS
TransIT-VirusGEN® SDS (PDF)
VirusGEN® AAV Transfection Kit SDS (PDF)
VirusGEN® LV Transfection Kit SDS (PDF)

Additional Resources

Virus Titer Reference Card: Methods to Measure Lentivirus and Adeno-associated Virus in Your Sample (PDF)

For a lot-specific Certificate of Analysis (COA) or Certificate of Origin (COO) containing a TSE/BSE Statement for this product, please contact techsupport@mirusbio.com.

FAQs

See the TransIT-VirusGEN FAQs

See the VirusGEN AAV FAQs

See the VirusGEN LV FAQs

Using transfection reagents and enhancers from Mirus has given our platform a competitive advantage. Increasing efficiency and lowering costs for all of our porgrams.

 

Sally Mader, PhD
ZYZ Theraputics