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Puromycin Dihydrochloride Antibiotic Solution

SKU MIR 5940 Category

$302.00

Description

Mirus Bio offers high quality cell culture grade selection antibiotics with the following features:

  • Ease of use – Sterile filtered ready-to-use stock solutions for stable cell line generation
  • High Purity – Greater than 90% purity determined by HPLC
  • High Potency – In combination with high purity ensures better selection at optimal concentrations

 

Stable cell line generation allows for sustained expression of an exogenous gene which is commonly enriched and maintained through the use of selection antibiotics such as G418, Hygromycin B, Puromycin, etc. Resistance to these antibiotics is conferred through the expression of a positive selection gene: neomycin resistance (neo), hygromycin resistance (hph) and puromycin-N-acetyl-transferase (pac) genes, respectively.

Selection markers can be delivered using the same plasmid (in cis) that contains the gene of interest, or on a separate plasmid (in trans) that needs to be co-transfected with the plasmid containing the gene of interest. For a detailed protocol, please visit our Stable cell line generation web page.

 

 

Puromycin for Stable Cell Line Selection

 

Puromycin for Stable Cell Line Selection. Puromycin is an aminoglycoside antibiotic that is used for the positive selection and maintenance of mammalian cells transfected with vectors that express puromycin-N-acetyl-transferase (PAC). This occurs as only cells that have successfully integrated the PAC gene can express the positive selection marker. The optimum concentration for selection is cell-type dependent; performing a kill curve is recommended with each new cell type or different antibiotic lot. Recommended concentration for the selection of mammalian cells that are resistant to Puromycin 0.5 – 10 µg/ml for mammalian cells.

MIR 5940

Resources

Specifications

Storage Conditions
G418 Sulfate Antibiotic Solution: Store at 4°C
Hygromycin B Antibiotic Solution: Store at 4°C
Puromycin Dihydrochloride Antibiotic Solution: Store at -20°C

Product Guarantee
All Configurations: As labeled on the product

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

 

Technical Product Literature

Quick Reference Protocol
Puromycin Solution Quick Ref Protocol (PDF)

SDS
Puromycin Solution SDS (PDF)

Using transfection reagents and enhancers from Mirus has given our platform a competitive advantage. Increasing efficiency and lowering costs for all of our porgrams.

 

Sally Mader, PhD
ZYZ Theraputics