pOET Transfer Plasmids are designed for use with the flashBAC™ Baculovirus Expression System and TransIT®-Insect Transfection Reagent.
Features of pOET Transfer Plasmids include:
- Ease-of-use – Simple construction of recombinant virus when used with the flashBAC™ Baculovirus Expression System
- Versatility – Transfer plasmids are available for insect or mammalian cell expression
- Compatibility – Can be used with any baculovirus expression system that relies on recombination in insect cells at the polyhedrin locus
SKU: MIR 6152
pOET6 BacMam Transfer Plasmid Map. pOET6 BacMam is a baculovirus transfer plasmid designed for use with the flashBAC™ Baculovirus Expression System and TransIT®-Insect Transfection Reagent. The CMV promoter enables high level gene expression in mammalian cells. pOET6 is compatible with any baculovirus expression system that utilizes homologous recombination at the polyhedrin locus.
flashBAC™ DNA Features: flashBAC™ DNA contains a bacterial artificial chromosome (BAC) that enables manufacturing of recombinant virus DNA in E. coli. lef2 and ORF1629 sequences flanking the BAC (in the flashBAC™ DNA) or gene of interest (in the transfer vector) allow for efficient homologous recombination in insect cells. A deletion in ORF1629 prevents replication of parental virus in insect cells. Deletion of the chitinase gene (chiA) maximizes production of secreted proteins and membrane-targeted proteins in insect cells.
Construction of Recombinant Virus Using flashBAC™: For baculovirus production, flashBAC™ DNA is mixed with transfer vector plasmid DNA containing the gene of interest to be inserted into the virus genome. Following co-transfection into insect cells, homologous recombination simultaneously removes the BAC, inserts the gene of interest and and restores ORF1629 allowing the production of infectious virus which can be harvested from the culture medium. Recombinant baculovirus can be used for subsequent transduction of insect or mammalian cells for protein expression.
flashBAC™ Systems and pOET vectors are sold by Mirus Bio through partnership with Oxford Expression Technologies, Oxford, UK.
All Configurations: Store at -20°C
All Configurations: 1 year
All Configurations: For Research Use Only.
Animal Origin Statement
All Configurations: This product is animal origin free.
Technical Product Literature
Product Data Sheet
pOET6 BacMam Plasmid Product Data Sheet (PDF)
pOET Plasmid SDS (PDF)