pOET1C_6xHis Transfer Plasmid – 20 ul (500 ng/ul)

Description

pOET Transfer Plasmids are designed for use with the flashBAC™ Baculovirus Expression System and TransIT®-Insect Transfection Reagent.

Features of pOET Transfer Plasmids include:

Ease-of-use – Simple construction of recombinant virus when used with the flashBAC™ Baculovirus Expression System
Versatility – Transfer plasmids are available for insect or mammalian cell expression
Compatibility – Can be used with any baculovirus expression system that relies on recombination in insect cells at the polyhedrin locus

SKU: MIR 6151

Supporting Data

pOET1C_6XHis Transfer Plasmid Map. pOET1C_6XHis, officially pOET1.1C_6XHis, is a baculovirus transfer plasmid is designed for use with the flashBAC™ Baculovirus Expression System and TransIT®-Insect Transfection Reagent. The powerful AcMNPV polyhedrin promoter affords high level gene expression in insect cells. A C-terminal 6×His-tag fusion sequence is included for recombinant protein purification with Ni-NTA agarose columns and subsequent removal by thrombin cleavage. pOET1C_6XHis is compatible with any baculovirus expression system that utilizes homologous recombination at the polyhedrin locus.

flashBAC™ DNA Features: flashBAC™ DNA contains a bacterial artificial chromosome (BAC) that enables manufacturing of recombinant virus DNA in E. coli. lef2 and ORF1629 sequences flanking the BAC (in the flashBAC™ DNA) or gene of interest (in the transfer vector) allow for efficient homologous recombination in insect cells. A deletion in ORF1629 prevents replication of parental virus in insect cells. Deletion of the chitinase gene (chiA) maximizes production of secreted proteins and membrane-targeted proteins in insect cells.

Construction of Recombinant Virus Using flashBAC™: For baculovirus production, flashBAC™ DNA is mixed with transfer vector plasmid DNA containing the gene of interest to be inserted into the virus genome. Following co-transfection into insect cells, homologous recombination simultaneously removes the BAC, inserts the gene of interest and and restores ORF1629 allowing the production of infectious virus which can be harvested from the culture medium. Recombinant baculovirus can be used for subsequent transduction of insect or mammalian cells for protein expression.

flashBAC™ Systems and pOET vectors are sold by Mirus Bio through partnership with Oxford Expression Technologies, Oxford, UK.

Resources

Specifications

Storage Conditions
All Configurations: Store at -20°C

Product Guarantee
All Configurations: 1 year

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

Technical Product Literature

Product Data Sheet
pOET1C_6xHis Plasmid Product Data Sheet (PDF)

SDS
pOET Plasmid SDS (PDF)

FAQs

See the flashBAC Baculovirus Expression FAQs

pOET1C_6xHis Transfer Plasmid – 20 ul (500 ng/ul)

For high yield protein production in insect and mammalian cells

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