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pLIVE Vector Complete System – 20 ug of each vector

A vector system designed to achieve long term expression in the livers of laboratory mice

SKU MIR 5320 Category

$854.00

Description

  • Sustained Gene Expression – Achieve long term liver gene expression in the mouse liver through 8 months post-injection
  • Versatile Platform – Available with positive control vectors expressing either LacZ or SEAP

Maps

Sequences

The pLIVE® Vector: DOWNLOAD PDF (PDF)

The pLIVE®-LacZ Vector: DOWNLOAD PDF (PDF)

The pLIVE®-SEAP Vector: DOWNLOAD PDF (PDF)

 

 

 

The Promoter in the pLIVE® Vector Outperforms the CMV Promoter for Long-term Liver Expression.

 

The Promoter in the pLIVE® Vector Outperforms the CMV Promoter for Long-term Liver Expression. The pLIVE®-SEAP Vector (20 µg) and pCMV-SEAP (10 µg, CMV promoter-driven SEAP vector) were each delivered to four ICR mice using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR 5240). At the indicated days post-injection, serum from each mouse was collected and the level of SEAP activity present was determined using the Phospha-Light™ SEAP Assay Kit (Applied Biosystems). The average SEAP activity from each group is presented.

 

 

 

Long-term Expression of Human Placental Secreted Alkaline Phosphatase (SEAP) in Mice after Hydrodynamic Delivery of the pLIVE®-SEAP Vector.

 

Long-term Expression of Human Placental Secreted Alkaline Phosphatase (SEAP) in Mice after Hydrodynamic Delivery of the pLIVE®-SEAP Vector. The pLIVE®-SEAP Vector was delivered to three C57Bl/6 mice using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR 5240). At the indicated times post-injection, serum from each mouse was collected and the level of SEAP activity was determined using the Phospha-Light™ SEAP Assay Kit (Applied Biosystems). Average SEAP activity at each time point is presented.

Strong Liver Expression of ß-galactosidase from pLIVE®-lacZ Vector after Hydrodynamic Tail Vein Injection.

Strong Liver Expression of ß-galactosidase from pLIVE®-lacZ Vector after Hydrodynamic Tail Vein Injection. The pLIVE®-lacZ Vector (Panel A) was delivered to an ICR mouse using the hydrodynamic tail vein injection procedure and the TransIT®-QR Hydrodynamic Delivery Solution (MIR 5240). At 24 hours post-injection, the liver was harvested, sectioned and stained with X-gal to demonstrate ß-galactosidase activity (blue cells). The cells were then counterstained with hematoxylin and eosin Y to stain the nuclei and cytoplasms, respectively. The control mouse (lacZ negative) in Panel B was stained in parallel to Panel A and contains no detectable ß-galactosidase activity.

 

SKU: MIR 5320

 

Resources

Specifications

Storage Conditions
All Configurations: Store vector DNA at -20°C. Prior to use, thaw vector DNA at room temperature, vortex, and quick spin in a microcentrifuge.

Product Guarantee
All Configurations: 1 year

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

 

Technical Product Literature

Full Protocols
pLIVE® Vectors Full Protocol (PDF)

SDS
pLIVE® Vectors SDS (PDF)

Using transfection reagents and enhancers from Mirus has given our platform a competitive advantage. Increasing efficiency and lowering costs for all of our porgrams.

 

Sally Mader, PhD
ZYZ Theraputics