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MiraCLEAN® Endotoxin Removal Kit – for 10 mg DNA

A convenient and effective method for the removal of endotoxin from plasmid DNA preparations

 

SKU MIR 5910 Categories ,

$212.00

Description

  • Effectively Removes Endotoxin from Plasmid Samples – Aids in the safety and efficiency of gene delivery research.
  • Compatible With In Vivo and In Vitro Applications – Removes harmful endotoxin that could cause decreased transfection efficiencies in vitro and inflammatory reactions in vivo.
  • Ease of Use – Simple phase separation protocol allows quick visualization of recovered nucleic acid preparations.

 

MiraCLEAN® Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per mg of DNA (EU/mg). The DNA was treated with the MiraCLEAN® Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.

 

Plasmid DNA Exhibits High Transfection Efficiency After Treatment with MiraCLEAN® Kit. COS-7 cells were transfected with 2 µg of plasmid DNA and 2µl of the TransIT®-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression.

Resources

Specifications

Storage Conditions
All Configurations: Store at 4°C

Product Guarantee
All Configurations: 1 year

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

Technical Product Literature

Full Protocol
MiraCLEAN® Full Protocol (PDF)

Quick Reference Protocol
MiraCLEAN® Quick Ref Protocol (PDF)

SDS
MiraCLEAN® SDS (PDF)

Using transfection reagents and enhancers from Mirus has given our platform a competitive advantage. Increasing efficiency and lowering costs for all of our programs.

Sally Mader, PhD
ZYZ Theraputics