Description
- Effectively Removes Endotoxin from Plasmid Samples – Aids in the safety and efficiency of gene delivery research.
- Compatible With In Vivo and In Vitro Applications – Removes harmful endotoxin that could cause decreased transfection efficiencies in vitro and inflammatory reactions in vivo.
- Ease of Use – Simple phase separation protocol allows quick visualization of recovered nucleic acid preparations.
MiraCLEAN® Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per mg of DNA (EU/mg). The DNA was treated with the MiraCLEAN® Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.
Plasmid DNA Exhibits High Transfection Efficiency After Treatment with MiraCLEAN® Kit. COS-7 cells were transfected with 2 µg of plasmid DNA and 2µl of the TransIT®-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression.