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Label IT Tracker Intracellular Nucleic Acid Localization Kit, Cy5 – labels 50-200 µg plasmid DNA

Efficient, direct labeling of plasmid DNA for in vitro and in vivo tracking experiments

SKU MIR 7021 Categories ,

$308.00

Description

  • Versatile Labeling – Efficiently label and visualize the DNA of your choice
  • One-step Chemical Method – Easily and consistently control the labeling reactions
  • High Efficiency Labeling – Optimal visualization of DNA in cells
  • Superior Tracking – Monitor both subcellular localization and reporter transgene expression following transfection of labeled (plasmid) DNA

 

Kit Components:
Each Kit contains Label IT® Tracker™ Reagent, Label IT® Reconstitution Solution, and 10X Labeling Buffer.

 

 

SKU: MIR 7021

Supporting Data

 

LabelIT Tracker Schematic Data

Label IT® Tracker™ Kits. The Label IT® Tracker™ labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with plasmid DNA and the reactive alkylating group (blue) that covalently attaches the Label IT® reagents to any reactive heteroatom within the plasmid. Attachment of the Label IT® Reagent to plasmids does not alter their structure or affect downstream gene expression, and as such, the same labeled plasmid can be both visualized after transfection by fluorescence microscopy and employed to express the gene of interest on the plasmid.

Label IT siRNA Tracker Intracellular Localization Kit Cy5 Time lapse tracking Data

Tracking of Plasmid Localization and Expression. COS-7 cells transfected with Label IT® Tracker™ Cy®5 (blue) labeled pEYFP-nuc and TransIT®-LT1 Reagent in serum containing media. Images were acquired at 3, 8, and 20 hours post-transfection. The blue signal indicates the cellular localization of the labeled plasmid, the green signal indicates cellular autofluorescence, and the yellow signal is the expression of the nuclear yellow fluorescent protein (YFP) reporter.

Resources

Specifications

Storage Conditions
All Configurations: Store the Label IT® Reagent at -20°C in both its dried pellet and reconstituted form. Store all other supplied reagents at -20°C. Warm kit components to room temperature and quick spin before each use.

Product Guarantee
All Configurations: 1 year (6 months after reconstitution)

Usage Statement
All Configurations: For Research Use Only.

Animal Origin Statement
All Configurations: This product is animal origin free.

 

Technical Product Literature

Full Protocol
Label IT Tracker™ Kit Full Labeling Protocol (PDF)

Quick Reference Protocol
Label IT Tracker™ Kit Quick Ref Protocol (PDF)

SDS
Label IT Tracker™ Kit SDS (PDF)

 

Additional Resources

Technical Report: Delivery Tools for Neurobiology (PDF)
Technical Report: Intracellular Localization and Expression (PDF)

FAQs

See the Label IT FAQs

Using transfection reagents and enhancers from Mirus has given our platform a competitive advantage. Increasing efficiency and lowering costs for all of our porgrams.

 

Sally Mader, PhD
ZYZ Theraputics